SMAC/Diablo Antibody

Western Blot: SMAC/Diablo Antibody - Unpurified [NB100-56166] - Analysis of A) MCF7 and B) K562 cell lysate using SMAC/Diablo this antibody at 1:500.

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody - Unpurified [NB100-56166] - Formalin-fixed, paraffin-embedded human colon stained for Smac/DIABLO using this antibody at 1:2000. A) normal colon. B) and C) colon carcinoma. Only trace Smac/DIABLO immunoreactivity is seen in normal colonic epithelium A), which is seen in the apical, foveolar cells. In contrast, strong immunoreactivity is observed in B) nuclear and C) cytoplasmic locations in the colon carcinoma specimen. Hematoxylin-eosin counterstain.

Western Blot: SMAC/Diablo Antibody - Unpurified [NB100-56166] - Analysis of Smac/DIABLO human tissues and cell lines using this antibody. Legend. SMAC: full-length recombinant smac. RS1846 and Jurkat are tumor cell lines. All tissues are from normal donors except colon carcinoma (Colon Ca). SMAC expression was detected in all samples except spleen and normal colon.

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody - Unpurified [NB100-56166] - Formalin-fixed, paraffin-embedded tissue section of human plasmacytoma (a mature B cell lymphoma) stained for Smac using this antibody at 1:2000. Intense nuclear staining is observed. Hematoxylin-eosin counterstain.

Product Discontinued

SMAC/Diablo Antibody Summary

• Immunogen: Full-length recombinant human SMAC was used as immunogen.
• Specificity: Smac
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DIABLO
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for SMAC/Diablo Antibody

• 0610041G12Rik
• Diablo
• diablo, IAP-binding mitochondrial protein
• Direct IAP-binding protein with low pI
• mitochondrial Smac protein
• Second mitochondria-derived activator of caspase
• SMAC
• SMACmitochondrial

Background

Smac/DIABLO (second mitochondrial-derived activator of caspase/direct IAP binding protein with low pI) is encoded by the nucleus and resides as a precursor within the intermembranous space of the mitochondria (reviewed in Ren et al, 2006; Shiozaki and Shi, 2004). During apoptosis, Smac/DIABLO is proteolytically cleaved and released from the mitochondria into the cytosol where it promotes cell death by interacting with IAPs and inhibiting their anti-apoptotic function. In turn, certain IAPs may degrade Smac/DIABLO and block its cell death function. Smac/DIABLO is expressed in a wide range of normal tissues and some tumor cells lines, and its over-expression in tumor cell lines can trigger both extrinsic and intrinsic apoptosis pathways. However, the expression and role of Smac/DIABLO in primary human tumors remains to be fully elucidated. Several alternatively spliced transcript variants that encode distinct SMAC isoforms have been described, including isoform 1 (approx. 27 kDa), isoform 2 (approx. 22 kDa) and isoform 3 (approx. 21 kDa).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SMAC/Diablo Antibody (NB100-56166)(2)

Publications using NB100-56166

• Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human)
  
  Details:
  Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).
• Krajewska M, Kim H, Kim C et al. Analysis of apoptosis protein expression in early-stage colorectal cancer suggests opportunities for new prognostic biomarkers. Clin Cancer Res. 2005-08-01 [PMID: 16061861] (IHC-P, WB, Human)
  
  Details:
  IHC-paraffin: Fig. 1F, WB: Fig. 3C.

Bioinformatics

• Gene Symbol: DIABLO
• Entrez:
  • 66593(Mouse)
  • 288753(Rat)