Western Blot: SMAC/Diablo Antibody [NB100-56166] - analysis of MCF7 (A) and K562 (B) cell lysate using SMAC/Diablo (NB100-56166) antibody at 1:500.
Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NB100-56166] - Formalin-fixed, paraffin-embedded human colon stained for Smac/DIABLO using NB100-56166 at 1:2000. A, normal colon. B and C, colon carcinoma. Only ...read more
Western Blot: SMAC/Diablo Antibody [NB100-56166] - Analysis of Smac/DIABLO human tissues and cell lines using this antibody. Legend. SMAC: full-length recombinant smac. RS1846 and Jurkat are tumor cell lines. All ...read more
Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NB100-56166] - Formalin-fixed, paraffin-embedded tissue section of human plasmacytoma (a mature B cell lymphoma) stained for Smac using NB100-56166 at 1:2000. ...read more
Hu, Mu, RtSpecies Glossary
WB, IHC, IHC-P, IP
This product is unpurified. The exact concentration of antibody is not quantifiable.
Smac/DIABLO (second mitochondrial-derived activator of caspase/direct IAP binding protein with low pI) is encoded by the nucleus and resides as a precursor within the intermembranous space of the mitochondria (reviewed in Ren et al, 2006; Shiozaki and Shi, 2004). During apoptosis, Smac/DIABLO is proteolytically cleaved and released from the mitochondria into the cytosol where it promotes cell death by interacting with IAPs and inhibiting their anti-apoptotic function. In turn, certain IAPs may degrade Smac/DIABLO and block its cell death function. Smac/DIABLO is expressed in a wide range of normal tissues and some tumor cells lines, and its over-expression in tumor cell lines can trigger both extrinsic and intrinsic apoptosis pathways. However, the expression and role of Smac/DIABLO in primary human tumors remains to be fully elucidated. Several alternatively spliced transcript variants that encode distinct SMAC isoforms have been described, including isoform 1 (approx. 27 kDa), isoform 2 (approx. 22 kDa) and isoform 3 (approx. 21 kDa).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.