SMAC/Diablo Antibody

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NB100-56164] - Formalin-fixed, paraffin-embedded tissue cores from a human breast cancer microarray stained for Smac/Diablo using NB100-56164 at 1:2000. Hematoxylin-eosin counterstain. Differential Smac/DIABLO staining is seen between patient samples.

Product Discontinued

SMAC/Diablo Antibody Summary

• Immunogen: A synthetic peptide corresponding to amino acids 56-73 (AVPIAQKSEPHSLSSEAL) of Smac/DIABLO was used as immunogen, GenBank no. NP_063940.1.
• Specificity: Smac
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DIABLO
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for SMAC/Diablo Antibody

• 0610041G12Rik
• Diablo
• diablo, IAP-binding mitochondrial protein
• Direct IAP-binding protein with low pI
• mitochondrial Smac protein
• Second mitochondria-derived activator of caspase
• SMAC
• SMACmitochondrial

Background

Smac/DIABLO (second mitochondrial-derived activator of caspase/direct IAP binding protein with low pI) is encoded by the nucleus and resides as a precursor within the intermembranous space of the mitochondria (reviewed in Ren et al, 2006; Shiozaki and Shi, 2004). During apoptosis, Smac/DIABLO is proteolytically cleaved and released from the mitochondria into the cytosol where it promotes cell death by interacting with IAPs and inhibiting their anti-apoptotic function. In turn, certain IAPs may degrade Smac/DIABLO and block its cell death function. Smac/DIABLO is expressed in a wide range of normal tissues and some tumor cells lines, and its over-expression in tumor cell lines can trigger both extrinsic and intrinsic apoptosis pathways. However, the expression and role of Smac/DIABLO in primary human tumors remains to be fully elucidated. Several alternatively spliced transcript variants that encode distinct SMAC isoforms have been described, including isoform 1 (approx. 27 kDa), isoform 2 (approx. 22 kDa) and isoform 3 (approx. 21 kDa).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DIABLO