SMAC/Diablo Antibody [HRP]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: HRP

SMAC/Diablo Antibody [HRP] Summary

• Immunogen: E. coli-derived recombinant human SMAC/Diablo
  Ala56-Asp239
  Accession # Q9NR28
• Specificity: Detects human, mouse, and rat SMAC/Diablo in direct ELISAs and Western blots. No cross-reactivity with recombinant human NME3 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DIABLO
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for SMAC/Diablo Antibody [HRP]

• 0610041G12Rik
• Diablo
• diablo, IAP-binding mitochondrial protein
• Direct IAP-binding protein with low pI
• mitochondrial Smac protein
• Second mitochondria-derived activator of caspase
• SMAC
• SMACmitochondrial

Background

Smac/DIABLO is a mitochondrial protein which activates various forms of apoptosis. Specifically, Smac exits the mitochondria and enters the cytosol where it initiates apoptosis by neutralizing members of the IAP family of apoptosis inhibitory proteins. Mitochondrial-mediated apoptosis is important in animal development and tissue homeostasis, with alterations resulting in a range of malignant disorders. Upon apoptotic stimuli, the mitochondrial proteins cytochrome c and Smac/DIABLO are released into the cytosol. The release of these proteins, however, occurs via different mechanisms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DIABLO