SMAC/Diablo Antibody (SC67-06)

Western Blot: SMAC/Diablo Antibody (SC67-06) [NBP2-67619] - Analysis of DIABLO on different lysates using anti-DIABLO antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: MCF-7

Immunocytochemistry/Immunofluorescence: SMAC/Diablo Antibody (SC67-06) [NBP2-67619] - Staining DIABLO in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody (SC67-06) [NBP2-67619] - Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-SMAC/Diablo antibody. Counter stained with hematoxylin.

Flow Cytometry: SMAC/Diablo Antibody (SC67-06) [NBP2-67619] - Flow cytometric analysis of HepG2 cells with SMAC/Diablo antibody at 1/50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: SMAC/Diablo Antibody (SC67-06) [NBP2-67619] - Staining DIABLO in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: SMAC/Diablo Antibody (SC67-06) [NBP2-67619] - Staining DIABLO in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: SMAC/Diablo Antibody (SC67-06) [NBP2-67619] - Staining DIABLO in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody (SC67-06) [NBP2-67619] - Analysis of paraffin-embedded human spleen tissue using anti-DIABLO antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody (SC67-06) [NBP2-67619] - Analysis of paraffin-embedded mouse overy tissue using anti-DIABLO antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody (SC67-06) [NBP2-67619] - Analysis of paraffin-embedded mouse skin tissue using anti-DIABLO antibody. Counter stained with hematoxylin.

Product Discontinued

SMAC/Diablo Antibody (SC67-06) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human SMAC/Diablo aa 190-239 / 239. (SwissProt: Q9NR28 Human; SwissProt: Q9JIQ3 Mouse; Unigene: 9090 Rat)
• Localization: Mitochondrion.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DIABLO
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SMAC/Diablo Antibody (SC67-06)

• 0610041G12Rik
• Diablo
• diablo, IAP-binding mitochondrial protein
• Direct IAP-binding protein with low pI
• mitochondrial Smac protein
• Second mitochondria-derived activator of caspase
• SMAC
• SMACmitochondrial

Background

Smac/DIABLO is a mitochondrial protein which activates various forms of apoptosis. Specifically, Smac exits the mitochondria and enters the cytosol where it initiates apoptosis by neutralizing members of the IAP family of apoptosis inhibitory proteins. Mitochondrial-mediated apoptosis is important in animal development and tissue homeostasis, with alterations resulting in a range of malignant disorders. Upon apoptotic stimuli, the mitochondrial proteins cytochrome c and Smac/DIABLO are released into the cytosol. The release of these proteins, however, occurs via different mechanisms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DIABLO