Recombinant Human SLPI Protein

Images

 
Western Blot: SLPI Protein [NBP1-45264] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human SLPI Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 26-132 of Human SLPI

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSGKSFKAGV CPPKKSAQCL RYKKPECQSD WQCPGKKRCC PDTCGIKCLD PVDTPNPTRR KPGKCPVTYG QCLMLNPPNF CEMDGQCKRD LKCCMGMCGK SCVSPVKA

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
SLPI
Purity
>80%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 2mM DTT, 100mM NaCl
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>80%, by SDS-PAGE

Alternate Names for Recombinant Human SLPI Protein

  • ALK1
  • ALPProtease inhibitor WAP4
  • antileukoproteinase
  • BLPISeminal proteinase inhibitor
  • HUSI
  • HUSI-I
  • Mucus proteinase inhibitor
  • secretory leukocyte peptidase inhibitor
  • secretory leukocyte protease inhibitor (antileukoproteinase)
  • Secretory leukocyte protease inhibitor
  • SLPI
  • WAP four-disulfide core domain protein 4
  • WAP4HUSI-1
  • WFDC4MPI

Background

SLPI is a secreted inhibitor which protects epithelial tissues from serine proteases. It is found in various secretions including seminal plasma, cervical mucus, and bronchial secretions, and has affinity for trypsin, leukocyte elastase, and cathepsin G. Its inhibitory effect contributes to the immune response by protecting epithelial surfaces from attack by endogenous proteolytic enzymes; the protein is also thought to have broad-spectrum anti-biotic activity. Recombinant human SLPI, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol SLPI
Entrez