SLPI Antibody (20409) [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clone: 20409
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

SLPI Antibody (20409) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human SLPI
  Accession # P03973
• Specificity: Detects recombinant human SLPI in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant mouse SLPI is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLPI
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SLPI Antibody (20409) [Unconjugated]

• ALK1
• ALPProtease inhibitor WAP4
• antileukoproteinase
• BLPISeminal proteinase inhibitor
• HUSI
• HUSI-I
• Mucus proteinase inhibitor
• secretory leukocyte peptidase inhibitor
• secretory leukocyte protease inhibitor (antileukoproteinase)
• Secretory leukocyte protease inhibitor
• SLPI
• WAP four-disulfide core domain protein 4
• WAP4HUSI-1
• WFDC4MPI

Background

SLPI is a member of the Serpin family and is primarily expressed in secretory glandular epithelial cells. It is found in various secretory fluids and functions as a potent inhibitor of many proteolytic enzymes. SLPI can modulate cell proliferation and has been shown to have anti-inflammatory, anti-viral, and anti-bacterial activities (1, 2).

1. Zhang, D. et al. (2002) J. Biol. Chem. 277:29999.
2. Taggart, C.C. et al. (2002) J. Biol. Chem. 277:33648.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLPI Antibody (MAB1274)(6)

Publications using MAB1274

• S Nugteren, Y Simons-Oos, CL Menckeberg, DH Hulleman-v, DJ Lindenberg, JN Samsom Endogenous secretory leukocyte protease inhibitor (SLPI) inhibits microbial-induced monocyte activation European Journal of Immunology, 2022-12-22;0(0):. 2022-12-22 [PMID: 36480463] (ELISA Capture, Human)
• Alexander C. Sun, Chengyang Yao, Venkatesh Venkatesh, Drew A. Hall An efficient power harvesting mobile phone-based electrochemical biosensor for point-of-care health monitoring Sensors and Actuators B: Chemical 11/1/2016 [PMID: 27725788]
• Alexander C. Sun, Chengyang Yao, Venkatesh Venkatesh, Drew A. Hall An efficient power harvesting mobile phone-based electrochemical biosensor for point-of-care health monitoring Sensors and Actuators B: Chemical 2016-11-01 [PMID: 27725788] (Bioassay, N/A)
• Austin W. Boesch, Yifeng Zhao, Alison S. Landman, Marta Rodriguez Garcia, John V. Fahey, Charles R. Wira et al. A multiplexed assay to detect antimicrobial peptides in biological fluids and cell secretions Journal of Immunological Methods 11/29/2013 [PMID: 24035708]
• Austin W. Boesch, Yifeng Zhao, Alison S. Landman, Marta Rodriguez Garcia, John V. Fahey, Charles R. Wira et al. A multiplexed assay to detect antimicrobial peptides in biological fluids and cell secretions Journal of Immunological Methods 2013-11-29 [PMID: 24035708] (ELISA Capture, Human)
• Gronborg M, Kristiansen TZ, Iwahori A, Chang R, Reddy R, Sato N, Molina H, Jensen ON, Hruban RH, Goggins MG, Maitra A, Pandey A Biomarker discovery from pancreatic cancer secretome using a differential proteomic approach. Mol. Cell Proteomics, 2005-10-08;5(1):157-71. 2005-10-08 [PMID: 16215274] (Western Blot, Human)

Bioinformatics

• Gene Symbol: SLPI
• Uniprot:
  • P03973()