SLP-76/LCP2 Antibody (SLP-76/03) - BSA Free

Western Blot: SLP-76/LCP2 Antibody (SLP-76/03) [NB500-537] - Lane 1: HPB-ALL human peripheral blood T cell leukemia cell line Lane 2: JURKAT human peripheral blood T cell leukemia cell line Lane 3: RAMOS human Burkitt lymphoma cell line

• Reactivity: Hu, Mu, Po
• Applications: WB, IHC
• Clone: SLP-76/03
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SLP-76/LCP2 Antibody (SLP-76/03) - BSA Free Summary

• Description: Novus Biologicals Mouse SLP-76/LCP2 Antibody (SLP-76/03) - BSA Free (NB500-537) is a monoclonal antibody validated for use in IHC and WB. Anti-SLP-76/LCP2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Bacterially expressed fusion protein representing amino acids 216-434 of human SLP76 with histidine tag
• Specificity: The polyclonal antibody reacts with SLP76, a 76kDa cytosolic adaptor protein that is involved in signaling of various hematopoietic cells, such as T cells, mast cells or neutrophils; in B cells, however, it is replaced by SLP65.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LCP2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1:1000
• Application Notes: Predicted molecular weight: 64 kDa.
• Theoretical MW: 76 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for SLP-76/LCP2 Antibody (SLP-76/03) - BSA Free

• LCP2
• lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of76kDa)
• lymphocyte cytosolic protein 2 (SH2 domain-containing leukocyte protein of76kD)
• lymphocyte cytosolic protein 2
• SH2 domain-containing leukocyte protein of 76 kDa
• SH2 domain-containing leukocyte protein of 76kD
• SLP-76 tyrosine phosphoprotein
• SLP76
• SLP-76
• SLP7676 kDa tyrosine phosphoprotein

Background

SLP76 (SH2 domain-containing leukocyte protein of 76 kDa) is a cytosolic adaptor protein which translocates to the plasma mambrane and is involved in multiple signaling pathways in T cells, mast cells, neutrophils and platelets; B cells express its analog SLP65/BLNK (B cell linker protein). SLP76 is phosphorylated by Syk-family and Tec-family tyrosine kinases and couples them to the phosphorylation and activation of PLC-gamma. Via Gads or Grb2, SLP76 also associates with LAT adaptor by involvement of SLP76 proline-rich region. The SH2 domain of SLP76 has been identified as the region involved in binding the serine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator by promoting the Jnk-mitogen activated protein kinase (MAPK) pathway and inhibiting the pathway leading to AP-1 activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLP-76/LCP2 Antibody (NB500-537)(1)

Publication using NB500-537

Bioinformatics

• Gene Symbol: LCP2
• Entrez:
  • 16822(Mouse)