SLP-76/LCP2 Antibody (SLP-76/03)

Images

 
Western Blot: SLP-76/LCP2 Antibody (SLP-76/03) [NB500-537] - Lane 1: HPB-ALL human peripheral blood T cell leukemia cell line Lane 2: JURKAT human peripheral blood T cell leukemia cell line Lane 3: RAMOS human Burkitt ...read more

Product Details

Summary
Reactivity Hu, Mu, PoSpecies Glossary
Applications WB, IHC, IHC-P
Clone
SLP-76/03
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

SLP-76/LCP2 Antibody (SLP-76/03) Summary

Immunogen
Bacterially expressed fusion protein representing amino acids 216-434 of human SLP76 with histidine tag
Specificity
The polyclonal antibody reacts with SLP76, a 76kDa cytosolic adaptor protein that is involved in signaling of various hematopoietic cells, such as T cells, mast cells or neutrophils; in B cells, however, it is replaced by SLP65.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
LCP2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
Application Notes
Predicted molecular weight: 64 kDa.
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NB500-537.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for SLP-76/LCP2 Antibody (SLP-76/03)

  • LCP2
  • lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of76kDa)
  • lymphocyte cytosolic protein 2 (SH2 domain-containing leukocyte protein of76kD)
  • lymphocyte cytosolic protein 2
  • SH2 domain-containing leukocyte protein of 76 kDa
  • SH2 domain-containing leukocyte protein of 76kD
  • SLP-76 tyrosine phosphoprotein
  • SLP76
  • SLP-76
  • SLP7676 kDa tyrosine phosphoprotein

Background

SLP76 (SH2 domain-containing leukocyte protein of 76 kDa) is a cytosolic adaptor protein which translocates to the plasma mambrane and is involved in multiple signaling pathways in T cells, mast cells, neutrophils and platelets; B cells express its analog SLP65/BLNK (B cell linker protein). SLP76 is phosphorylated by Syk-family and Tec-family tyrosine kinases and couples them to the phosphorylation and activation of PLC-gamma. Via Gads or Grb2, SLP76 also associates with LAT adaptor by involvement of SLP76 proline-rich region. The SH2 domain of SLP76 has been identified as the region involved in binding the serine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator by promoting the Jnk-mitogen activated protein kinase (MAPK) pathway and inhibiting the pathway leading to AP-1 activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Po
Applications: WB, IHC, IHC-P

Publications for SLP-76/LCP2 Antibody (NB500-537)(1)


Showing Publication 1 - 1 of 1.
Publication using NB500-537 Applications Species

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for SLP-76/LCP2 Antibody (NB500-537) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB500-537AF350
Alexa Fluor 405 NB500-537AF405
Alexa Fluor 488 NB500-537AF488
Alexa Fluor 532 NB500-537AF532
Alexa Fluor 594 NB500-537AF594
Alexa Fluor 647 NB500-537AF647
Alexa Fluor 700 NB500-537AF700
Alexa Fluor 750 NB500-537AF750
Biotin NB500-537B
DyLight 350 NB500-537UV
DyLight 405 NB500-537V
DyLight 488 NB500-537G
DyLight 550 NB500-537R
DyLight 594 NB500-537DL594
DyLight 650 NB500-537C
DyLight 680 NB500-537FR
DyLight 755 NB500-537IR
FITC NB500-537F
HRP NB500-537H
Janelia Fluor 549 NB500-537JF549
Janelia Fluor 646 NB500-537JF646

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Diseases for SLP-76/LCP2 Antibody (NB500-537)

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Bioinformatics

Gene Symbol LCP2
Entrez