SLP-76/LCP2 Antibody (460004)

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human SLP-76/LCP2 Monoclonal Antibody (Catalog # MAB5637) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SLP-76/LCP2 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 460004
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

SLP-76/LCP2 Antibody (460004) Summary

• Immunogen: E. coli-derived recombinant human SLP-76
  Met1-Pro533
  Accession # Q13094
• Specificity: Detects endogenous human SLP-76 in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LCP2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SLP-76/LCP2 Antibody (460004)

• LCP2
• lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of76kDa)
• lymphocyte cytosolic protein 2 (SH2 domain-containing leukocyte protein of76kD)
• lymphocyte cytosolic protein 2
• SH2 domain-containing leukocyte protein of 76 kDa
• SH2 domain-containing leukocyte protein of 76kD
• SLP-76 tyrosine phosphoprotein
• SLP76
• SLP-76
• SLP7676 kDa tyrosine phosphoprotein

Background

SLP-76 is a 533 amino acid (aa), 76 kDa tyrosine phosphorylated intracellular docking protein with a single SH2 recognition domain. It is expressed in T cells, platelets, neutrophils, NK and mast cells, and is required for progression of T cells to double positive stage in the thymus. Tyrosine phosphorylation is required for interaction with the signaling proteins VAV, NCK, PLC gamma , ITK, and ZAP70. SLP-76 may be phosphorylated by ZAP70 leading to NF-AT and IL2 gene activation. Human SLP-76 shares 83% and 85% aa sequence identity with mouse and rat SLP-76, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LCP2
• Uniprot:
  • Q13094()