SLC7A8 Antibody (OTI5A9)

Western Blot: SLC7A8 Antibody (5A9) [NBP1-47990] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC7A8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC7A8.

Immunocytochemistry/Immunofluorescence: SLC7A8 Antibody (5A9) [NBP1-47990] Staining of COS7 cells transiently transfected by pCMV6-ENTRY SLC7A8.

Immunohistochemistry-Paraffin: SLC7A8 Antibody (5A9) [NBP1-47990] - Staining of paraffin-embedded Human pancreas tissue using anti-SLC7A8 mouse monoclonal antibody.

Flow Cytometry: SLC7A8 Antibody (5A9) [NBP1-47990] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SLC7A8 antibody, and then analyzed by flow cytometry.

Immunoprecipitation: SLC7A8 Antibody (5A9) [NBP1-47990] - Lane 1: Negative Control Lane 2: NBP1-47990 Lane 3: NBP1-47988 Lane 4: NBP2-02092 Lane 5: NBP2-02109 Lane 6: NBP2-02110

Product Discontinued

SLC7A8 Antibody (OTI5A9) Summary

• Immunogen: Protein expressed in 293T cell transfected with human SLC7A8 expression vector.
• Specificity: This antibody is specific for Homo sapiens solute carrier family 7 (amino acid transporter, L-type), member 8 (SLC7A8), transcript variant 1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC7A8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 2ug/500ul

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SLC7A8 Antibody (OTI5A9)

• hLAT2
• integral membrane protein E16H
• large neutral amino acids transporter small subunit 2
• LAT2L-type amino acid transporter 2
• LPI-PC1
• solute carrier family 7 (amino acid transporter, L-type), member 8
• Solute carrier family 7 member 8
• y+ system), member 8

Background

Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine orD,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC7A8
• Entrez:
  • 23428(Human)