SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free

Western Blot: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] - Boiled and unboiled HeLa whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (NBP3-25549) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Immunohistochemistry-Paraffin: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] - LAT1 / SLC7A5 antibody [HL2353] detects LAT1 / SLC7A5 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. LAT1 / SLC7A5 stained by LAT1 / SLC7A5 antibody [HL2353] (NBP3-25549) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (NBP3-25549) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] - LAT1 / SLC7A5 antibody [HL2353] detects LAT1 / SLC7A5 protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: LAT1 / SLC7A5 stained by LAT1 / SLC7A5 antibody [HL2353] (NBP3-25549) diluted at 1:500. Blue: Fluoroshield with DAPI .

Unboiled various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SLC7A5/LAT1 antibody [HL2353] (NBP3-25549) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SLC7A5/LAT1 antibody [HL2353] (NBP3-25549) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SLC7A5/LAT1 antibody [HL2353] (NBP3-25549) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

SLC7A5/LAT1 antibody [HL2353] detects SLC7A5/LAT1 protein at cell membrane by immunofluorescent analysis.Sample: Caco-2 cells were fixed in ice-cold MeOH for 5 min.Green: SLC7A5/LAT1 stained by SLC7A5/LAT1 antibody [HL2353] (NBP3-25549) diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: HL2353
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free (NBP3-25549) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human SLC7A5/LAT1. The exact sequence is proprietary.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SLC7A5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free

• 4F2 LC
• 4F2LC
• CD98
• CD98lc
• D16S469EL-type amino acid transporter 1
• E16
• Integral membrane protein E16
• large neutral amino acids transporter 1
• large neutral amino acids transporter small subunit 1
• LAT1
• LAT1hLAT1
• MPE16
• MPE16CD98 light chain
• SLC7A5
• sodium-independent neutral amino acid transporter LAT1,4F2LC
• solute carrier family 7 (cationic amino acid transporter, y+ system), member 5,4F2 light chain
• Solute carrier family 7 member 5
• TA1
• y+ system cationic amino acid transporter

Background

Mammalian cells have several amino acid transport systems. These systems have been defined by both activity and specific genes. L-type amino acid transporter 1 (LAT1) is a 12 membrane-spanning protein. LAT1 is a Na + -independent neutral amino acid transporter agency and essential for the transporter of large neutral amino acid such as Leucine, Isoleucine, and Valine through the plasma membrane. LAT1 has been proposed to be one of the major nutrient transport systems at the blood-brain barrier. Drugs such as L Dopa are transported by LAT1. LAT1 is thought to be up-regulated to support the high protein synthesis for cell growth in some tumor cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC7A5