SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free [NBP3-20116] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of EP3-1 (NBP3-20116) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20116, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Flow Cytometry: SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free [NBP3-20116] - Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of EP3-1 (NBP3-20116, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

• Reactivity: Hu
• Applications: Flow, Func, ICC/IF, IP
• Clone: EP3-1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free (NBP3-20116) is a recombinant monoclonal antibody validated for use in Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Immunised mice and constructed IgG1 Fab phage display libraries from the spleen cells. SLC7A5/LAT1-reactive recombinant Fab fragments were retrieved by epitope peptide panning.
• Epitope: Shown to bind to epitopes of sequences; LLHGDFHAFSAG-GGGC-NH2, LLHGDFH-GGGC-HN2, and FHAFSAG-GGGC-NH2.
• Specificity: Shows no reactivity to live HeLa cells by ICC/IF.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SLC7A5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Functional
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free

• 4F2 LC
• 4F2LC
• CD98
• CD98lc
• D16S469EL-type amino acid transporter 1
• E16
• Integral membrane protein E16
• large neutral amino acids transporter 1
• large neutral amino acids transporter small subunit 1
• LAT1
• LAT1hLAT1
• MPE16
• MPE16CD98 light chain
• SLC7A5
• sodium-independent neutral amino acid transporter LAT1,4F2LC
• solute carrier family 7 (cationic amino acid transporter, y+ system), member 5,4F2 light chain
• Solute carrier family 7 member 5
• TA1
• y+ system cationic amino acid transporter

Background

Mammalian cells have several amino acid transport systems. These systems have been defined by both activity and specific genes. L-type amino acid transporter 1 (LAT1) is a 12 membrane-spanning protein. LAT1 is a Na + -independent neutral amino acid transporter agency and essential for the transporter of large neutral amino acid such as Leucine, Isoleucine, and Valine through the plasma membrane. LAT1 has been proposed to be one of the major nutrient transport systems at the blood-brain barrier. Drugs such as L Dopa are transported by LAT1. LAT1 is thought to be up-regulated to support the high protein synthesis for cell growth in some tumor cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC7A5