SLC7A5/LAT1 Antibody (BU53) [Alexa Fluor® 647]

Flow (Cell Surface): SLC7A5/LAT1 Antibody (BU53) [Alexa Fluor 647] [NBP2-50465AF647] - A cell surface stain was performed on Raji cells with SLC7A5 (BU53) antibody NBP2-50465AF647 (blue) along with a matched isotype control NB600-986AF647 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at RT. Both antibodies were conjugated to Alexa Fluor 647.

• Reactivity: Hu
• Applications: Flow, IHC, CyTOF-ready
• Clone: BU53
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

SLC7A5/LAT1 Antibody (BU53) [Alexa Fluor® 647] Summary

• Immunogen: Plasma cell line 8226
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC7A5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Cell Surface)
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for SLC7A5/LAT1 Antibody (BU53) [Alexa Fluor® 647]

• 4F2 LC
• 4F2LC
• CD98
• CD98lc
• D16S469EL-type amino acid transporter 1
• E16
• Integral membrane protein E16
• large neutral amino acids transporter 1
• large neutral amino acids transporter small subunit 1
• LAT1
• LAT1hLAT1
• MPE16
• MPE16CD98 light chain
• SLC7A5
• sodium-independent neutral amino acid transporter LAT1,4F2LC
• solute carrier family 7 (cationic amino acid transporter, y+ system), member 5,4F2 light chain
• Solute carrier family 7 member 5
• TA1
• y+ system cationic amino acid transporter

Background

Mammalian cells have several amino acid transport systems. These systems have been defined by both activity and specific genes. L-type amino acid transporter 1 (LAT1) is a 12 membrane-spanning protein. LAT1 is a Na + -independent neutral amino acid transporter agency and essential for the transporter of large neutral amino acid such as Leucine, Isoleucine, and Valine through the plasma membrane. LAT1 has been proposed to be one of the major nutrient transport systems at the blood-brain barrier. Drugs such as L Dopa are transported by LAT1. LAT1 is thought to be up-regulated to support the high protein synthesis for cell growth in some tumor cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLC7A5/LAT1 Antibody (NBP2-50465AF647)(2)

Publications using NBP2-50465AF647

• Juliana Cazarin, Rachel E. DeRollo, Siti Noor Ain Binti Ahmad Shahidan, Jamison B. Burchett, Daniel Mwangi, Saikumari Krishnaiah et al. MYC disrupts transcriptional and metabolic circadian oscillations in cancer and promotes enhanced biosynthesis PLOS Genetics 2023-08-28 [PMID: 37639465] (Human)
• Tan SY, Kelkar Y, Hadjipanayis A et al. Metformin and 2-Deoxyglucose Collaboratively Suppress Human CD4+ T Cell Effector Functions and Activation-Induced Metabolic Reprogramming J. Immunol. 2020-07-08 [PMID: 32641388] (Human)
  
  Details:
  Citation using the Alexa Fluor 647 format of this antibody.

Bioinformatics

• Gene Symbol: SLC7A5