SLC6A3/DAT1 Antibody (CL3123) - Azide and BSA Free

Staining of human caudate nucleus shows strong positivity in dopaminergic fibers.

Staining of human cerebral cortex shows weak positivity in a subset of neuronal processes.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Staining of rat caudate putamen shows moderate positivity in dopaminergic fibers.

Staining of mouse caudate putamen shows moderate positivity in dopaminergic fibers.

Staining of human substantia nigra shows strong cytoplasmic positivity in dopaminergic neurons.

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clone: CL3123
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

SLC6A3/DAT1 Antibody (CL3123) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse SLC6A3/DAT1 Antibody (CL3123) - Azide and BSA Free (NBP2-46649) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q01959, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC6A3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for SLC6A3/DAT1 Antibody (CL3123) - Azide and BSA Free

• DA transporter
• DAT1
• DATDAT1Solute carrier family 6 member 3
• SLC6A3
• sodium-dependent dopamine transporter
• solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
• VNTR

Background

The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for SLC6A3/DAT1 Antibody (NBP3-43803). (Showing 1 - 1 of 1 FAQ).

1. Could you kindly provide published references for Western blot in detection of Dopamine transporter in human brain tissue with your antibody/antibodies?
   • Catalog numbers NBP2-22164, NB300-254 and NB100-65459 are well characterized DAT1 antibodies that have been referenced. If you refer to our website we have links to the PMID listing for each reference on the individual datasheets.

Bioinformatics

• Gene Symbol: SLC6A3