SLC6A15 Overexpression Lysate (Native)

Images

 
Western Blot: SLC6A15 Overexpression Lysate (Adult Normal) [NBL1-16179] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC6A15.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SLC6A15 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC209725 and based on accession number NM_182767. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens solute carrier family 6 (neutral amino acid transporter), member 15 (SLC6A15), transcript variant 1, mRNA.
Gene
SLC6A15

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
81.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SLC6A15

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SLC6A15 Overexpression Lysate (Native)

  • B0AT2
  • DKFZp761I0921
  • FLJ10316
  • homolog of rat orphan transporter v7-3
  • hv7-3
  • MGC87066
  • NTT73orphan sodium- and chloride-dependent neurotransmitter transporter NTT73
  • orphan transporter v7-3
  • SBAT1
  • Sodium- and chloride-dependent neurotransmitter transporter NTT73
  • sodium/chloride dependent neurotransmitter transporter Homo sapiens orphanneurotransmitter transporter NTT7
  • Sodium-coupled branched-chain amino-acid transporter 1
  • solute carrier family 6 (neurotransmitter transporter), member 15
  • solute carrier family 6 (neutral amino acid transporter), member 15
  • Solute carrier family 6 member 15
  • solute carrier family 6, member 15
  • Transporter v7-3
  • V7-3

Background

SLC6A15 shows structural characteristics of an Na(+) and Cl(-)-dependent neurotransmitter transporter, including 12 transmembrane (TM) domains, intracellular N and C termini, and large extracellular loops containing multiple N-glycosylation sites (Farmer et al., 2000 [PubMed 11112352]).[supplied by OMIM]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol SLC6A15