MKP-3/DUSP6 Antibody (3G2)

Images

 
Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of DUSP6 expression in transfected 293T cell line by DUSP6 monoclonal antibody (M01), clone 3G2.Lane 1: DUSP6 transfected lysate(42 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of monoclonal antibody to DUSP6 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of monoclonal antibody to DUSP6 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.
Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - DUSP6 monoclonal antibody (M01), clone 3G2 Western Blot analysis of DUSP6 expression in Jurkat ( Cat # L017V1 ).
Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - DUSP6 monoclonal antibody (M01), clone 3G2. Western Blot analysis of DUSP6 expression in human placenta.
Genetic Strategies: Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of DUSP6 over-expressed 293 cell line, cotransfected with DUSP6 Validated Chimera RNAi ( Cat # H00001848-R01V ) (Lane 2) or ...read more
ELISA: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Detection limit for recombinant GST tagged DUSP6 is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, RNAi, S-ELISA
Clone
3G2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

MKP-3/DUSP6 Antibody (3G2) Summary

Immunogen
DUSP6 (AAH03143, 1 a.a. ~ 381 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MIDTLRPVPFASEMAISKTVAWLNEQLELGNERLLLMDCRPQELYESSHIESAINVAIPGIMLRRLQKGNLPVRALFTRGEDRDRFTRRCGTDTVVLYDESSSDWNENTGGESLLGLLLKKLKDEGCRAFYLEGGFSKFQAEFSLHCETNLDGSCSSSSPPLPVLGLGGLRISSDSSSDIESDLDRDPNSATDSDGSPLSNSQPSFPVEILPFLYLGCAKDSTNLDVLEEFGIKYILNVTPNLPNLFENAGEFKYKQIPISDHWSQNLSQFFPEAISFIDEARGKNCGVLVHCLAGISRSVTVTVAYLMQKLNLSMNDAYDIVKMKKSNISPNFNFMGQLLDFERTLGLSSPCDNRVPAQQLYFTTPSNQNVYQVDSLQST
Localization
Cytoplasmic
Specificity
DUSP6 - dual specificity phosphatase 6
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DUSP6
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • RNA Inhibition
  • Sandwich ELISA 1:100-1:2000
Application Notes
Antibody reactivity against recominant protein, tissue lysate, transfected lysate and cell lysate for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA.
Control
MKP-3/DUSP6 Knockout HeLa Cell Lysate
Publications
Read Publications using
H00001848-M01 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MKP-3/DUSP6 Antibody (3G2)

  • dual specificity phosphatase 6
  • Dual specificity protein phosphatase PYST1
  • DUSP6
  • EC 3.1.3.16
  • EC 3.1.3.48
  • MAP kinase phosphatase 3
  • Mitogen-activated protein kinase phosphatase 3
  • MKP3
  • MKP-3
  • MKP3serine/threonine specific protein phosphatase
  • PYST1
  • PYST1dual specificity protein phosphatase 6

Background

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MKP-3/DUSP6 Antibody (H00001848-M01)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MKP-3/DUSP6 Antibody (H00001848-M01) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DUSP6
Entrez
OMIM
Uniprot