SLC5A5/Sodium Iodide Symporter Antibody (FP5)

Images

 
Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - analysis of Human thyroid lysate showing detection of Sodium Iodide Symporter protein using Mouse Anti-Sodium Iodide Symporter Monoclonal ...read more
Immunohistochemistry-Paraffin: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium ...read more
Immunohistochemistry-Paraffin: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium ...read more
Immunohistochemistry-Paraffin: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Tissue: Trachea. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
FP5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

SLC5A5/Sodium Iodide Symporter Antibody (FP5) Summary

Immunogen
Mapped to AA 625-643 of hNIS. Apparent mol. wt of 97kD, non-glycosylated version at 68kD. Other minor bands associated with hNIS at160kDa, and degradation products at approx. 30 kDa, and approx. 15kDa.Mannose binding protein hNIS fusion (AA468-643).
Localization
Membrane
Specificity
Detects approx 97kDa, non-glycosylated version at 68kDa. Other minor bands associated with hNIS at 160kDa, and degradation products at approx 30 kDa, and approx 15kDa.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SLC5A5
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
Application Notes
1 ug/ml of Sodium-Iodide Symporter Antibody was sufficient for detection of hNIS in 20 ug of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Publications
Read Publications using NBP1-70342.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for SLC5A5/Sodium Iodide Symporter Antibody (FP5)

  • Na(+)/I(-) cotransporter
  • Na(+)/I(-) symporter
  • NIS
  • NISNa(+)/I(-)-symporter
  • SLC5A5
  • sodium/iodide cotransporter
  • Sodium-iodide symporter
  • solute carrier family 5 (sodium iodide symporter), member 5
  • Solute carrier family 5 member 5
  • TDH1

Background

The sodium iodide symporter (NIS) is an ion pump that actively transports iodide across the basolateral membrane into thyroid epithelial cells (1, 2). This is important step in the process of iodide organificaton and the formation of triiodothyronine and thyroxine (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SLC5A5/Sodium Iodide Symporter Antibody (NBP1-70342)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-70342AF405
Alexa Fluor 488 Labeled NBP1-70342AF488
Alexa Fluor 647 Labeled NBP1-70342AF647
Alexa Fluor 700 Labeled NBP1-70342AF700
DyLight 350 Labeled NBP1-70342UV
DyLight 405 Labeled NBP1-70342V
DyLight 488 Labeled NBP1-70342G
DyLight 680 Labeled NBP1-70342FR
DyLight 755 Labeled NBP1-70342IR

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Bioinformatics

Gene Symbol SLC5A5
Entrez
Uniprot