SLC16A3 Antibody

Product Discontinued

SLC16A3 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 241-290 of Human MCT4.
• Specificity: MCT4 (F265) detects endogenous levels of MCT4 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC16A3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of MCT4 (F265) pAb in extracts from LOVO cells.
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SLC16A3 Antibody

• MCT 3
• MCT3
• MCT3MCT 4
• MCT4
• MCT4MGC138472
• MGC138474
• Monocarboxylate transporter 3
• monocarboxylate transporter 4
• SLC16A3
• solute carrier family 16 (monocarboxylic acid transporters), member 3
• Solute carrier family 16 member 3
• solute carrier family 16, member 3 (monocarboxylic acid transporter 4)

Background

Monocarboxylates, such as lactate and pyruvate, play an integral role in cellular metabolism. Lactic acid is produced in large quantities as a result of glycolysis, which provides the majority of ATP to cells under normal physiological conditions. However, accumulation of lactic acid leads to a decrease in intracellular pH and cessation of glycolysis. In order for glycolysis to continue at a high rate, lactic acid must be transported out of the cell. This transport process is carried out by a family of monocarboxylate transporters (MCTs),which function as proton symports and are stereoselective for L-lactate.The MCT family consists of at least eight members, MCT1-8, which contain between 10-12 transmembrane-helical (TM) domains, with the amino and carboxy termini located in the cytoplasm. MCT1 is widely expressed and is the major form of MCTs in tumor cells and erythrocytes. MCT2 is highly expressed in liver and testis, while MCT3 and MCT4 are predominantly expressed in skeletal muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLC16A3 Antibody (NBP1-00920)(4)

Publications using NBP1-00920

• Nishioku T, Anzai R, Hiramatsu S et al. Lactate dehydrogenase A inhibition prevents RANKL-induced osteoclastogenesis by reducing enhanced glycolysis Journal of Pharmacological Sciences 2023-12-01 [PMID: 37973217] (WB, Mouse)
• Tsai SF, Wu HT, Chen PC et al. High-fat diet suppresses the astrocytic process arborization and downregulates the glial glutamate transporters in the hippocampus of mice. Brain Res. 2018-07-13 [PMID: 30009766] (WB, Mouse)
• Tsai SF, Ku NW, Wang TF et al. Long-Term Moderate Exercise Rescues Age-Related Decline in Hippocampal Neuronal Complexity and Memory Gerontology 2018-05-07 [PMID: 29734165] (Mouse)
• Dovmark TH, Saccomano M, Hulikova A et al. Connexin-43 channels are a pathway for discharging lactate from glycolytic pancreatic ductal adenocarcinoma cells Oncogene 2017-04-04 [PMID: 28368405] (WB, Human)

Bioinformatics

• Gene Symbol: SLC16A3
• Entrez:
  • 9123(Human)
  • 80879(Mouse)
  • 80878(Rat)
• Uniprot:
  • O15427(Human)