SLC10A2 Antibody

Immunohistochemistry-Paraffin: SLC10A2 Antibody [NBP1-36987] - 3.8ug/ml Staining of Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SLC10A2 Antibody Summary

• Description: Novus Biologicals Goat SLC10A2 Antibody (NBP1-36987) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-SLC10A2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-YKANGGFQPDEK corresponding to C-Terminus according to NP_000443.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SLC10A2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1:100 - 1:2000
• Application Notes: WB: Preliminary experiments gave an approx. 60 kDa band in human kidney lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 37.7 kDa band according to NP_000443.1. The 60 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human small intestine shows staining of cells in the laminia propria between the intestinal vili.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SLC10A2 Antibody

• Apical sodium-dependent bile acid transporter
• ASBT
• IBAT
• ileal apical sodium-dependent bile acid transporter
• Ileal Na(+)/bile acid cotransporter
• ileal sodium/bile acid cotransporter
• Ileal sodium-dependent bile acid transporter
• ISBT
• Na(+)-dependent ileal bile acid transporter
• NTCP2
• NTCP2PBAM
• PBAM
• SLC10A2
• Sodium/taurocholate cotransporting polypeptide, ileal
• solute carrier family 10 (sodium/bile acid cotransporter family), member 2
• Solute carrier family 10 member 2

Background

SLC10A2 plays a critical role in the sodium-dependent reabsorption of bile acids from the lumen of the small intestine. Plays a key role in cholesterol metabolism

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLC10A2 Antibody (NBP1-36987)(1)

Publication using NBP1-36987

• Annaba F, Sarwar Z, Kumar P et al. Modulation of ileal bile acid transporter (ASBT) activity by depletion of plasma membrane cholesterol: association with lipid rafts. Am J Physiol Gastrointest Liver Physiol 2008-02-01 [PMID: 18063707]

Bioinformatics

• Gene Symbol: SLC10A2
• Entrez:
  • 6555(Human)
• Uniprot:
  • NP_000443.1(Human)