SLAM/CD150 Antibody (459911)

Mouse splenocytes were treated for 2 days with 50 ng/mL PMA and 500 ng/mL Ca2+ionomycin then stained with Mouse SLAM/CD150 Monoclonal Antibody (Catalog # MAB4330, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113).

• Reactivity: Mu
• Applications: Flow, CyTOF-ready, ELISA(Cap)
• Clone: 459911
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

SLAM/CD150 Antibody (459911) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse SLAM/CD150
  Thr25-Pro242
  Accession # Q9QUM4
• Specificity: Detects mouse SLAM/CD150.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: SLAMF1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 2.5 ug/10^6 cells
• Application Notes: ELISA Capture: Mouse SLAM/CD150 Antibody (Catalog # MAB4330)
  ELISA Detection: Mouse SLAM/CD150 Biotinylated Antibody (Catalog # BAF4330)
  Standard: Recombinant Mouse SLAM/CD150 (Catalog # 4330-SL)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SLAM/CD150 Antibody (459911)

• CD 150
• CD150
• CD150IPO-3
• CDw150
• IPO-3
• signaling lymphocytic activation molecule family member 1
• signaling lymphocytic activation molecule
• SLAM
• SLAMCD150 antigen
• SLAMF1

Background

The type I transmembrane glycoprotein Signaling Lymphocytic Activation Molecule (SLAM), also known as CD150, is the prototypic member of the SLAM subgroup of the CD2 protein family. CD2 family proteins function as adhesion molecules and modulators of the immune response (1). Mouse SLAM consists of a 218 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 78 aa cytoplasmic domain with three immunoreceptor tyrosine switch motifs (ITSM) (2). Alternate splicing generates an isoform with a substituted cytoplasmic domain (2). Within the ECD, mouse SLAM shares 58% and 83% aa sequence identity with human and rat SLAM, respectively. It is expressed as a 75 kDa molecule of which approximately 30 kDa is N-linked carbohydrate (2). SLAM is expressed on T cells, B cells, thymocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells (2‑7). It is upregulated on activated B cells and CD4⁺ and CD8⁺ T cells, although it is downregulated on Th2 polarized cells (2, 3, 8). SLAM interacts homophilically with low affinity, and this interaction induces a Th0/Th1 response characterized by clonal expansion, production of IFN-gamma , and increased cytolytic activity of CD8⁺ T cells (2, 3, 9‑11). SLAM ligation also promotes B cell activation, allergen-induced eosinophil and mast cell activation, and macrophage responsiveness to LPS (4, 8, 12). In humans, SLAM functions as a cellular entry receptor for measles virus (13, 14).

1. Ma, C.S. et al. (2007) Annu. Rev. Immunol. 25:337.
2. Castro, A.G. et al. (1999) J. Immunol. 163:5860.
3. Cocks, B.G. et al. (1995) Nature 376:260. 
4. Wang, N. et al. (2004) J. Exp. Med. 199:1255. 
5. Hahm, B. et al. (2004) Virology 323:292. 
6. Nanda, N. et al. (2005) Blood 106:3028. 
7. Kiel, M.J. et al. (2005) Cell 121:1109. 
8. Punnonen, J. et al. (1997) J. Exp. Med. 185:993.
9. Mavaddat, N. et al. (2000) J. Biol. Chem. 275:28100.
10. Aversa, G. et al. (1997) J. Immunol. 158:4036.
11. Mehrle, S. et al. (2008) Mol. Immunol. 45:796.
12. Wang, N. et al. (2006) Am. J. Respir. Cell Mol. Biol. 35:206.
13. Tatsuo, H. et al. (2000) Nature 406:893.
14. Hsu, E.C. et al. (2001) Virology 279:9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLAM/CD150 Antibody (MAB4330)(2)

Publications using MAB4330

• JA Bennett, KP Singh, SL Welle, LA Boule, BP Lawrence, TA Gasiewicz Conditional deletion of Ahr alters gene expression profiles in hematopoietic stem cells PLoS ONE, 2018-11-02;13(11):e0206407. 2018-11-02 [PMID: 30388136] (Flow Cytometry, Mouse)
• Wang L, Benedito R, Bixel M, Zeuschner D, Stehling M, Savendahl L, Haigh J, Snippert H, Clevers H, Breier G, Kiefer F, Adams R Identification of a clonally expanding haematopoietic compartment in bone marrow. EMBO J, 2012-11-27;32(2):219-30. 2012-11-27 [PMID: 23188081] (IHC, Mouse)

Bioinformatics

• Gene Symbol: SLAMF1
• Uniprot:
  • Q9QUM4()