SKA3 Antibody - BSA Free

Western Blot: SKA3 Antibody [NBP1-76311] - Human testis tissue lysate with SKA3 antibody at (A) 0.5 and (B) 1 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SKA3 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human SKA3. The immunogen is located within amino acids 290 - 340 of SKA3. Amino Acid Squence: STNYPLSKTNSSS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SKA3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 0.5-1 ug/ml
• Control Peptide:

  SKA3 Peptide (NBP1-76311PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for SKA3 Antibody - BSA Free

• spindle and kinetochore associated complex subunit 3
• spindle and kinetochore-associated protein 3

Background

Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Like with SKA1 or SKA2, depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged a metaphase-like state. These SKA3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, suggesting the SKA complex plays a key role in spindle checkpoint silencing and the maintenance of chromosome cohesion in mitosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SKA3
• Uniprot:
  • NP_659498(Human)