SIX5 Antibody - BSA Free

Western Blot: SIX5 Antibody - BSA Free [NBP3-45347] - Y79 cells were subjected to SDS PAGE followed by western blot with (SIX5 Antibody) at dilution of 1:600

Immunoprecipitation: SIX5 Antibody - BSA Free [NBP3-45347] - IP Result of anti-SIX5 (IP:, 4ug; Detection: 1:300) with Y79 cells lysate 2000ug.

Immunohistochemistry: SIX5 Antibody - BSA Free [NBP3-45347] - Immunohistochemistry of paraffin-embedded human kidney tissue slide using (SIX5 Antibody) at dilution of 1:200

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SIX5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SIX5 Antibody - BSA Free (NBP3-45347) is a polyclonal antibody validated for use in IHC, WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: SIX homeobox 5. (Uniprot# Q8N196)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIX5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:20-1:200
  • Immunoprecipitation 1:200-1:1000
  • Western Blot 1:500-1:2000
• Application Notes: Theoretical Molecular Weight is 100-105 kDa

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SIX5 Antibody - BSA Free

• BOR2
• DM locus-associated homeodomain protein
• DMAHPsine oculis homeobox homolog 5 (Drosophila)
• dystrophia myotonica-associated homeodomain protein
• homeobox protein SIX5
• sine oculis homeobox (Drosophila) homolog 5
• Sine oculis homeobox homolog 5
• SIX homeobox 5

Background

Six5 (homeobox protein SIX5), also known as SIX5, BOR2 or DMAHP (DM locus-associated homeodomain protein), is a transcription factor that is expressed in various structures of the adult eye. Localized to the cytoplasm in early development and to the nucleus in the later stages of development,Six5 is involved in regulation of organogenesis and in maintenance of retinal formation. Six5 is able to bind the 5'-TCA[AG][AG]TTNC-3' DNA sequence found in the myogenin and IGFBP5 promoters and, through this binding, can control transcription of the associated mRNA. Six5 is regulated via association with DACH1 (dachshund homolog 1) and is co-activated by the EYA (eyes absent) proteins. Defects in the gene encoding Six5 are the cause of branchiooto- renal syndrome type 2 (BOR2), an autosomal disorder characterized by hearing loss, a deep overbite and myopia. Two isoforms exist due to alternative splicing events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIX5