SIVA Overexpression Lysate (Native)

Western Blot: SIVA Overexpression Lysate (Adult Normal) [NBL1-15981] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIVA.

• Reactivity: Hu
• Applications: WB

SIVA Overexpression Lysate (Native) Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC203748 and based on accession number NM_021709. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens SIVA1, apoptosis-inducing factor (SIVA1), transcript variant 2, mRNA.
• Gene: SIVA1

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
• Theoretical MW: 11.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for SIVA

• Type: Overexpression
• Protein State: Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SIVA Overexpression Lysate (Native)

• CD27-binding (Siva) protein
• CD27BPCD27-binding protein
• Siva-1
• SIVA1, apoptosis-inducing factor
• Siva-2
• SIVAapoptosis regulatory protein Siva

Background

This gene encodes a protein with an important role in the apoptotic (programmed cell death) pathway induced by the CD27 antigen, a member of the tumor necrosis factor receptor (TFNR) superfamily. The CD27 antigen cytoplasmic tail binds to the N-terminus of this protein. Two alternatively spliced transcript variants encoding distinct proteins have been described. [provided by RefSeq]. Transcript Variant: This variant (2) lacks an alternate in-frame segment, compared to variant 1. It appears to lack the apoptotic properties of Siva. The resulting protein (isoform 2) is shorter when it is compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SIVA1