Recombinant Human Sirtuin 5/SIRT5 Protein

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SDS-Page: SIRT5 Protein [NBP2-22807]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human Sirtuin 5/SIRT5 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 34-310 of Human Sirtuin 5/SIRT5

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSARPSSSM ADFRKFFAKA KHIVIISGAG VSAESGVPTF RGAGGYWRKW QAQDLATPLA FAHNPSRVWE FYHYRREVMG SKEPNAGHRA IAECETRLGK QGRRVVVITQ NIDELHRKAG TKNLLEIHGS LFKTRCTSCG VVAENYKSPI CPALSGKGAP EPGTQDASIP VEKLPRCEEA GCGGLLRPHV VWFGENLDPA ILEEVDRELA HCDLCLVVGT SSVVYPAAMF APQVAARGVP VAEFNTETTP ATNRFRFHFQ GPCGTTLPEA LACHENETVS

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
SIRT5
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
32.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Sirtuin 5/SIRT5 Protein

  • EC 3.5.1
  • EC 3.5.1.-
  • FLJ36950
  • NAD-dependent deacetylase sirtuin-5
  • silent mating type information regulation 2, S.cerevisiae, homolog 5
  • SIR2L5
  • sir2-like 5
  • SIR2-like protein 5
  • SIRT5
  • sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
  • sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 5
  • Sirtuin 5
  • sirtuin type 5

Background

SIRT5 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants Recombinant human SIRT5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol SIRT5
Entrez