Sirtuin 5/SIRT5 Antibody (OTI2F1) [Alexa Fluor™ Plus 680]

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready, KO
• Clone: OTI2F1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 680

Sirtuin 5/SIRT5 Antibody (OTI2F1) [Alexa Fluor™ Plus 680] Summary

• Immunogen: Full length human recombinant protein of human SIRT5(NP_036373) produced in HEK293 cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIRT5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Sirtuin 5/SIRT5 Antibody (OTI2F1) [Alexa Fluor™ Plus 680]

• EC 3.5.1
• EC 3.5.1.-
• FLJ36950
• NAD-dependent deacetylase sirtuin-5
• silent mating type information regulation 2, S.cerevisiae, homolog 5
• SIR2L5
• sir2-like 5
• SIR2-like protein 5
• SIRT5
• sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
• sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 5
• Sirtuin 5
• sirtuin type 5

Background

SIRT5 encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in two transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIRT5