Sirtuin 2/SIRT2 Antibody - BSA Free

Images

 
Western Blot: Sirtuin 2/SIRT2 Antibody [NBP1-76879] - Mouse brain lysate with SIRT2 antibody at (A) 1 and (B) 2 ug/mL.
Immunohistochemistry: Sirtuin 2/SIRT2 Antibody - BSA Free [NBP1-76879] - Immunohistochemical staining of Human Brain tissue using Sirtuin 2/SIRT2 antibody at 2.5 u/mL.
Immunocytochemistry/ Immunofluorescence: Sirtuin 2/SIRT2 Antibody - BSA Free [NBP1-76879] - Immunofluorescence of Sirtuin 2/SIRT2 in Human Brain tissue with Sirtuin 2/SIRT2 antibody at 5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Sirtuin 2/SIRT2 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of the human SIRT2. The immunogen is located within the last 50 amino acids of SIRT2. Amino Acid Squence: SPKKSPPPAKDEARTTERE
Marker
Early Oligodendrocyte Marker
Predicted Species
Rabbit (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SIRT2
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 5 ug/mL
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Sirtuin 2/SIRT2 Peptide (NBP1-76879PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-76879 in the following applications:

Publications
Read Publication using
NBP1-76879 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for Sirtuin 2/SIRT2 Antibody - BSA Free

  • EC 3.5.1
  • EC 3.5.1.-
  • FLJ35621
  • FLJ37491
  • S.cerevisiae, homolog) 2
  • SIR2L2
  • SIRT2
  • Sirtuin 2
  • sirtuin type 2

Background

The founding member of the sirtuin protein family was the silent information regulator 2 protein (Sir2p) of Saccharomyces cervisiae, an NAD+-dependent histone deacetylase (HDAC) that regulates chromatin silencing. The SIR2 family of genes are highly conserved from prokaryotes to eukaryotes. Mammals have seven homologs of Sir2p, SIRT1-7, which are involved in diverse processes ranging from transcriptional regulation, cell cycle progression and DNA-damage repair to aging. SIRT2 is a predominantly cytoplasmic protein that colocalizes with microtubules and can deacetylate alpha-tubulin and regulate progression through the cell cycle. Most Sirtuins are highly expressed in brain and testis, while Sirt2 expression is higher in fetal relative to adult brain. Recent studies on SIRT2 support the therapeutic utility of inhibitors for the treatment of neurodegenerative diseases such as Parkinson's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Sirtuin 2/SIRT2 Antibody (NBP1-76879)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Review for Sirtuin 2/SIRT2 Antibody (NBP1-76879) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-76879:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Sirtuin 2/SIRT2 NBP1-76879
Enlarge
5
reviewed by:
Verified Customer
WB Human 12/18/2023
View

Summary

ApplicationWestern Blot
Sample TestedHepG2,Huh-7 human hepatoma cell line
SpeciesHuman
Lot4485-1003

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Sirtuin 2/SIRT2 Antibody (NBP1-76879) (0)

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Recent Reviews

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Verified Customer
12/18/2023
Application: WB
Species: Human

Bioinformatics

Gene Symbol SIRT2
Entrez
Uniprot