SIRP alpha/CD172a Antibody [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 532

SIRP alpha/CD172a Antibody [Alexa Fluor® 532] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human SIRPα/CD172a
  Gly27-Asn370
  Accession # P78324
• Specificity: Detects human SIRP alpha /CD172a in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SIRP alpha/CD172a Antibody [Alexa Fluor® 532]

• BIT
• BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs
• Brain Ig-like molecule with tyrosine-based activation motifs
• CD172 antigen-like family member A
• CD172a antigen
• CD172a
• Inhibitory receptor SHPS-1
• Macrophage fusion receptor
• MFR
• MFRtyrosine phosphatase SHP substrate 1
• MyD-1 antigen
• MYD1
• MYD-1
• p84
• protein tyrosine phosphatase, non-receptor type substrate 1
• PTPNS1
• SHP substrate 1
• SHPS1
• SHPS-1
• SHPS1CD172A
• signal-regulatory protein alpha
• Signal-regulatory protein alpha-1
• Signal-regulatory protein alpha-2
• Signal-regulatory protein alpha-3
• SIRP alpha
• SIRPA
• SIRPalpha
• SIRP-ALPHA-1
• SIRPalpha2
• Sirp-alpha-2
• Sirp-alpha-3
• SIRPtyrosine-protein phosphatase non-receptor type substrate 1

Background

Signal regulatory protein alpha (SIRP alpha , designated CD172a), also called SHPS-1 (SHP substrate 1) and previously, MyD-1 (Myeloid/Dendritic-1), is a monomeric ~90 kDa type I transmembrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin superfamily (1‑4). SIRPs are paired receptors, with similar extracellular domains but differing C-termini and functions (1, 2). The 503 amino acid (aa) human SIRP alpha contains a 342 aa extracellular domain (ECD), with one V-type, and two C1 type Ig domains, and three potential N glycosylation sites. It has a 110 aa cytoplasmic sequence with ITIM motifs that recruit tyrosine phosphatases SHP-1 and SHP-2 when phosphorylated (4). Human SIRP alpha has more than 40 described polymorphisms, including the prominent BIT (Brain Ig like molecule with Tyrosine-based activation motifs, also called SIRP alpha ₂ or PTPNS) (5). One reported isoform lacks aa 1‑101, which eliminates most of the V type Ig domain. Human SIRP alpha ECD shares 61%, 60%, 71%, 72% and 73% aa identity with mouse, rat, porcine, bovine and equine SIRP alpha , respectively; it shares 84% and 76% aa identity with human SIRP beta 1 and SIRP gamma , respectively (2). SIRP alpha is expressed mainly on myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells (3‑6). It is also found on neurons, smooth muscle and endothelial cells (7‑9). SIRP alpha shows adhesion to the ubiquitous CD47/IAP (integrin associated protein), while SIRP gamma binds more weakly and SIRP alpha 1 does not bind at all (1, 2). Mouse and human SIRP alpha -CD47 binding only cross-reacts for specific polymorphisms and influences engraftment of xenotransplanted stem cells (6, 10). SIRP alpha engagement generally produces a negative regulatory signal (4). Low SIRP alpha recognition of CD47, which occurs on aged erythrocytes or platelets or xenogenic cells, promotes clearance of CD47^low cells from circulation (11, 13). SIRP alpha recognition of surfactants SP-A and SP-D in the lung can inhibit alveolar macrophage cytokine production (14). The CD47 integrin-SIRP alpha interaction is reported to promote macrophage fusion during osteoclastogenesis (15).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.