SIRP alpha/CD172a Antibody (772734) [Allophycocyanin]

PC-12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Mouse/Rat SIRPa/CD172a APC-conjugated Monoclonal Antibody (Catalog # FAB7307A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Mouse splenocytes were stained with Rat Anti-Mouse Integrin aM/CD11b PE-conjugated Monoclonal Antibody (Catalog # FAB1124P) and either (A) Mouse Anti-Mouse/Rat SIRPa/CD172a APC-conjugated Monoclonal Antibody (Catalog # FAB7307A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu, Rt
• Applications: Flow
• Clone: 772734
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

SIRP alpha/CD172a Antibody (772734) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat SIRP alpha /CD172a
  Lys32-Asn373
  Accession # P97710
• Specificity: Detects rat SIRP alpha /CD172a in direct ELISAs and Western blots. Detects mouse and rat SIRP alpha /CD172a in flow cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIRPA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SIRP alpha/CD172a Antibody (772734) [Allophycocyanin]

• BIT
• BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs
• Brain Ig-like molecule with tyrosine-based activation motifs
• CD172 antigen-like family member A
• CD172a antigen
• CD172a
• Inhibitory receptor SHPS-1
• Macrophage fusion receptor
• MFR
• MFRtyrosine phosphatase SHP substrate 1
• MyD-1 antigen
• MYD1
• MYD-1
• P84
• protein tyrosine phosphatase, non-receptor type substrate 1
• PTPNS1
• SHP substrate 1
• SHPS1
• SHPS-1
• SHPS1CD172A
• signal-regulatory protein alpha
• Signal-regulatory protein alpha-1
• Signal-regulatory protein alpha-2
• Signal-regulatory protein alpha-3
• SIRP alpha
• SIRPA
• SIRPalpha
• Sirp-alpha-1
• SIRPalpha2
• Sirp-alpha-2
• Sirp-alpha-3
• SIRPtyrosine-protein phosphatase non-receptor type substrate 1

Background

SIRP alpha (Signal regulatory protein alpha; also known as CD172a, Shps1 and Bit) is a variably glycosylated, 85-120 kDa member of the SIRP ‘family' of proteins. It is expressed on neurons, macrophages, monocytes, granulocytes and dendritic cells. SIRP alpha is phosphorylated/activated in response to cell adhesion. This may, or may not, involve binding to known ligands CD47, SP-A and SP-D. Following phosphorylation, SIRP alpha binds to SHP-1 and -2, resulting in the negative regulation of immune system activity. Mature rat SIRP alpha is a 478 amino acid (aa) type I transmembrane glycoprotein. It contains a 342 aa extracellular region (aa 32-373) that possesses one V-type and two C1-type Ig-like domains. Its cytoplasmic domain possesses two ITIMs that interact with protein phosphatases. There is one potential splice variant that shows a four aa insertion after Gln424. Over aa 32-373, rat SIRP alpha shares 63% and 73% aa sequence identity with human and mouse SIRP alpha , respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIRPA
• Uniprot:
  • P97710()