SIRP alpha/CD172a Antibody (772734) [Allophycocyanin]

• Applications: Flow
• Clone: 772734
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

SIRP alpha/CD172a Antibody (772734) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat SIRP alpha /CD172a
  Lys32-Asn373
  Accession # P97710
• Specificity: Detects rat SIRP alpha /CD172a in direct ELISAs and Western blots. Detects mouse and rat SIRP alpha /CD172a in flow cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIRPA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

SIRP alpha (Signal regulatory protein alpha; also known as CD172a, Shps1 and Bit) is a variably glycosylated, 85-120 kDa member of the SIRP ‘family' of proteins. It is expressed on neurons, macrophages, monocytes, granulocytes and dendritic cells. SIRP alpha is phosphorylated/activated in response to cell adhesion. This may, or may not, involve binding to known ligands CD47, SP-A and SP-D. Following phosphorylation, SIRP alpha binds to SHP-1 and -2, resulting in the negative regulation of immune system activity. Mature rat SIRP alpha is a 478 amino acid (aa) type I transmembrane glycoprotein. It contains a 342 aa extracellular region (aa 32-373) that possesses one V-type and two C1-type Ig-like domains. Its cytoplasmic domain possesses two ITIMs that interact with protein phosphatases. There is one potential splice variant that shows a four aa insertion after Gln424. Over aa 32-373, rat SIRP alpha shares 63% and 73% aa sequence identity with human and mouse SIRP alpha , respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIRPA