SIN3B Antibody - BSA Free

Western Blot: SIN3B Antibody [NBP2-20368] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 5% SDS PAGE gel, diluted at 1:500.

Immunocytochemistry/Immunofluorescence: SIN3B Antibody [NBP2-20368] - SIN3B antibody detects SIN3B protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SIN3B protein stained by SIN3B antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Western Blot: SIN3B Antibody [NBP2-20368] - Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE gel, diluted at 1:1000.

Immunoprecipitation: SIN3B Antibody [NBP2-20368] - Immunoprecipitation of SIN3B protein from HCT-116 whole cell extracts using 5 µg of SIN3B antibody. Western blot analysis was performed using SIN3B antibody diluted at 1:500.

SIN3B antibody [N1N2-2], N-term detects SIN3B protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SIN3B protein stained by SIN3B antibody [N1N2-2], N-term (NBP2-20368) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 um.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SIN3B Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SIN3B Antibody - BSA Free (NBP2-20368) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human SIN3B. The exact sequence is proprietary.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIN3B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SIN3B Antibody - BSA Free

• Histone deacetylase complex subunit Sin3b
• KIAA0700paired amphipathic helix protein Sin3b
• SIN3 homolog B, transcription regulator (yeast)
• SIN3 homolog B, transcriptional regulator (yeast)
• SIN3 homolog B, transcriptional regulator
• Transcriptional corepressor Sin3b

Background

Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SIN3B
• Uniprot:
  • O75182(Human)