SIN3A Antibody (JA94-31)

Western Blot: SIN3A Antibody (JA94-31) [NBP2-67146] - Analysis of mSin3A on 293T cell using anti-mSin3A antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: SIN3A Antibody (JA94-31) [NBP2-67146] - Staining mSin3A in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SIN3A Antibody (JA94-31) [NBP2-67146] - Analysis of paraffin-embedded human kidney tissue using anti-mSin3A antibody. Counter stained with hematoxylin.

Flow Cytometry: SIN3A Antibody (JA94-31) [NBP2-67146] - Analysis of MCF-7 cells with mSin3A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Immunocytochemistry/Immunofluorescence: SIN3A Antibody (JA94-31) [NBP2-67146] - Staining mSin3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: SIN3A Antibody (JA94-31) [NBP2-67146] - Staining mSin3A in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

SIN3A Antibody (JA94-31) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human SIN3A aa 1224-1273 / 1273. (SwissProt: Q96ST3 Human; SwissProt: Q60520 Mouse; Entrez Gene: 363067 Rat)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SIN3A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SIN3A Antibody (JA94-31)

• DKFZp434K2235
• FLJ90319
• Histone deacetylase complex subunit Sin3a
• KIAA0700
• paired amphipathic helix protein Sin3a
• SIN3 homolog A, transcription regulator (yeast)
• SIN3 homolog A, transcriptional regulator (yeast)
• SIN3A
• Transcriptional corepressor Sin3a
• transcriptional co-repressor Sin3A
• transcriptional regulator, SIN3A

Background

Sin3A is encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIN3A