Siglec-8 Antibody

Western Blot: Siglec-8 Antibody [NBP1-06078] - (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Siglec-8 Antibody Summary

• Immunogen: Peptide with sequence C-SYKSQLNYKTKQ corresponding to internal region according to NP_055257.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SIGLEC8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 45 kDa band observed in MOLT4 lysates is reported in scientific literature (PMID: 12609831).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Siglec-8 Antibody

• CD329 antigen
• CDw329
• MGC59785
• SAF2
• SAF2SAF-2
• sialic acid binding Ig-like lectin 8
• Sialoadhesin family member 2
• Siglec8
• Siglec-8
• SIGLEC8L
• SIGLEC8Lsialic acid-binding Ig-like lectin 8

Background

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Siglec-8 Antibody (NBP1-06078)(2)

Publications using NBP1-06078

• Yokoi H, Choi OH, Hubbard W et al. Inhibition of FcepsilonRI-dependent mediator release and calcium flux from human mast cells by sialic acid-binding immunoglobulin-like lectin 8 engagement. J Allergy Clin Immunol 2008-02-01 [PMID: 18036650]
• Nutku E, Aizawa H, Hudson SA et al. Ligation of Siglec-8: a selective mechanism for induction of human eosinophil apoptosis. Blood 2003-06-01 [PMID: 12609831] (WB)

Bioinformatics

• Gene Symbol: SIGLEC8
• Entrez:
  • 27181(Human)
• Uniprot:
  • Q9NYZ4()