Siglec-10 Antibody - BSA Free

Western Blot: Siglec-10 Antibody [NBP1-82759] - Analysis in control (vector only transfected HEK293T lysate) and SIGLEC10 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: Siglec-10 Antibody [NBP1-82759] - Staining of human colon shows weak to moderate cytoplasmic/membranous positivity in lymphoid cells.

Immunohistochemistry-Paraffin: Siglec-10 Antibody [NBP1-82759] - Staining of human bone marrow shows strong cytoplasmic positivity in subsets of cells.

Immunohistochemistry-Paraffin: Siglec-10 Antibody [NBP1-82759] - Staining of human skeletal shows no positivity in myocytes as expected.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Siglec-10 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: DGRFWIRVQESVMVPEGLCISVPCSFSYPRQDWTGSTPAYGYWFKAVTETTKGAPVATNHQSREVEMSTRGRFQLTGDPAKGNCSLVIRDAQMQDESQY
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIGLEC10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Siglec-10 Protein (NBP1-82759PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Siglec-10 Antibody - BSA Free

• MGC126774
• PRO940
• sialic acid binding Ig-like lectin 10
• sialic acid-binding Ig-like lectin 10
• Siglec10
• Siglec-10
• siglec-like gene 2
• Siglec-like protein 2
• SLG2
• SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7

Background

SIGLEC10 - sialic acid binding Ig-like lectin 10

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Siglec-10 Antibody (NBP1-82759)(1)

Publication using NBP1-82759

• Zhang C, Zhang J, Liang F et al. Innate immune checkpoint Siglec10 in cancers: mining of comprehensive omics data and validation in patient samples Frontiers of medicine 2022-01-24 [PMID: 35075579]

Bioinformatics

• Gene Symbol: SIGLEC10