Siglec-G Antibody (805903)

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 805903
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Siglec-G Antibody (805903) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Siglec-G
  Glu20-Gln525
  Accession # NP_766488
• Specificity: Detects mouse Siglec-G in ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Siglec-F or recombinant human Siglec-10 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Siglecg
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-G Antibody (805903)

• PRO940;Sialic acid-binding Ig-like lectin 10;SIGLEC10;SIGLEC-10;SLG2
• Siglec-10
• SiglecG
• Siglec-G

Background

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by a V-type Ig-like domain which mediates sialic acid binding, followed by varying numbers of C2-type Ig-like domains. Mouse Siglec-G, the apparent ortholog of human Siglec-10, is a 110-120 kDa, 688 amino acid (aa) type I transmembrane protein mainly expressed on mouse B1-type B cells. It controls B1 cell survival, selection, expansion and calcium signaling by negatively regulating B cell receptor signals. A potentially secreted 269 aa variant diverges after the first two Ig-like domains. Mouse Siglec-G shares approximately 63% aa sequence identity with human Siglec-10 within the extracellular domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Siglecg
• Uniprot:
  • NP_766488()