Shugoshin Antibody [DyLight 405]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 405

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Catalog# & Conjugate Size Price

Shugoshin Antibody [DyLight 405] Summary

Immunogen
Synthetic peptide made to an internal portion of human Shugoshin (within residues 350-450). [Swiss-Prot# Q5FBB7]
Localization
Nucleus. Centromere. Note= Localizes to the centromere. BUB1 is required for centromeric localization. During prometaphase, it localizes to a single focus, while at metaphase, it localizes to 2 spots corresponding to the 2 centromeres.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SGOL1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This Shugoshin antibody is useful for Western blot, where a band is seen at ~64 kDa. In ICC/IF nuclear staining was observed in HeLa cells.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen sequence has 53% homology to Mouse's Shugoshin/SGOL1 protein.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Shugoshin Antibody [DyLight 405]

  • FLJ14230
  • hSgo1
  • NY-BR-85
  • Serologically defined breast cancer antigen NY-BR-85
  • SGO
  • Sgo1
  • shugoshin 1AB protein
  • shugoshin 1CD protein
  • shugoshin 1EF protein
  • shugoshin 1GH protein
  • shugoshin 1KL protein
  • shugoshin-like 1 (S. pombe)
  • shugoshin-like 1

Background

Shugoshin (SGOL) proteins provide essential protection to cohesin (a protein complex that hold sister chromatids together) from cleavage by separase enzyme during meiosis-I as well as from phosphorylation-mediated removal during mitotic cell division. SGOL forms a complex with phosphatase PP2A that dephosphorylates cohesion resulting in its retention at centromeres during the onset of meiotic anaphase and vertebrate mitotic prophase I. Shugoshin (Japanese for guardian spirit), was originally identified in budding/fission yeasts as protector of centromeric cohesion in meiosis and later on it was found to be highly conserved among eukaryotic organisms with mammalian SGOL family consisting of two members: SGOL1 and SGOL2. SGOL1 regulates accurate chromosome segregation during mitosis, whereas, SGOL2 plays a dual role in chromosome congression as well as centromeric protection of cohesion. Defects in chromosome cohesion system and mis-regulation of SGOL functions may lead to pathologies including cancer and infertility. SGO1 has been shown to implicate in human colon cancers and lentivirus-mediated siRNA interference targeting SGO-1 inhibits human NSCLC cell growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SGOL1