SHP-2/PTPN11 Antibody - BSA Free

Western Blot: SHP-2/PTPN11 Antibody [NBP2-42920] - Non-infected (-) and infected (+) BHK-21 whole cell extracts (15 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus NS1 protein antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: SHP-2/PTPN11 Antibody [NBP2-42920] - Analysis of Denque virus 2-infected BHK-21, using NS1 (Dengue virus) antibody at 1:2000 dilution.

• Reactivity: V-Vi
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SHP-2/PTPN11 Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of SHP-2/PTPN11 (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTPN11
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:1000-1:2000
  • Western Blot 1:500-1:3000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Dengue Virus.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for SHP-2/PTPN11 Antibody - BSA Free

• BPTP3
• EC 3.1.3.48
• MGC14433
• protein tyrosine phosphatase, non-receptor type 11
• protein tyrosine phosphatase-2
• Protein-tyrosine phosphatase 1D
• Protein-tyrosine phosphatase 2C
• PTP1D
• PTP-1D
• PTP2C
• PTP-2C
• PTP2CNS1
• PTPN11
• SHP2
• SHP-2
• SHP2CFC
• SHPTP2
• SH-PTP2
• SH-PTP2Noonan syndrome 1
• SH-PTP3
• tyrosine-protein phosphatase non-receptor type 11

Background

SHP-2 (also called PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp and PTPN11) is a protein tyrosine phosphatase (PTP) expressed in most cell types (unlike SHP-1), (1). SHP-2 is involved in t the signal transduction stimulated by EGF, PDGF and Insulin. After cell stimulation, SHP-2 (as well as SHP-1), translocates from cytosol to the plasma membrane and binds to tyrosine-phosphorylated receptors through their SH2 domains, becoming activated in the process (2). When phosphorylated, SHP-2 has been shown to bind Grb2, EGFR, PDGFR, IRS-1 via its SH2 domain (3). Over expression of SHP-2 leads to Juvenile Myelo-monocytic Leukemia and Noonan syndrome (4-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPN11