SHP-2/PTPN11 Antibody (M163)

Western Blot: SHP-2/PTPN11 Antibody (M163) [NBP3-23400] - Western blot analysis of adult mouse brain. The blot was probed with anti-SHP2 (N-terminal) antibody at 1:250 (lane 1), 1:500 (lane 2), 1:1000 (lane 3), and 1:2000 (lane 4).

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: M163
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SHP-2/PTPN11 Antibody (M163) Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Clone (M163) was generated from a recombinant protein containing amino acids in the N-terminal region of human SHP-2/PTPN11. This sequence is highly conserved in rat and mouse SHP-2/PTPN11. [UniProt# Q06124]
• Specificity: The antibody detects a 72 kDa protein in human A431 and Jurkat cells, and mouse brain. This antibody does not cross-react with SHP1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPN11
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 72 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for SHP-2/PTPN11 Antibody (M163)

• BPTP3
• EC 3.1.3.48
• MGC14433
• protein tyrosine phosphatase, non-receptor type 11
• protein tyrosine phosphatase-2
• Protein-tyrosine phosphatase 1D
• Protein-tyrosine phosphatase 2C
• PTP1D
• PTP-1D
• PTP2C
• PTP-2C
• PTP2CNS1
• PTPN11
• SHP2
• SHP-2
• SHP2CFC
• SHPTP2
• SH-PTP2
• SH-PTP2Noonan syndrome 1
• SH-PTP3
• tyrosine-protein phosphatase non-receptor type 11

Background

SHP-2 (also called PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp and PTPN11) is a protein tyrosine phosphatase (PTP) expressed in most cell types (unlike SHP-1), (1). SHP-2 is involved in t the signal transduction stimulated by EGF, PDGF and Insulin. After cell stimulation, SHP-2 (as well as SHP-1), translocates from cytosol to the plasma membrane and binds to tyrosine-phosphorylated receptors through their SH2 domains, becoming activated in the process (2). When phosphorylated, SHP-2 has been shown to bind Grb2, EGFR, PDGFR, IRS-1 via its SH2 domain (3). Over expression of SHP-2 leads to Juvenile Myelo-monocytic Leukemia and Noonan syndrome (4-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPN11