SHMT1 Overexpression Lysate

Western Blot: SHMT1 Overexpression Lysate (Adult Normal) [NBL1-15947] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SHMT1.

• Reactivity: Hu
• Applications: WB

SHMT1 Overexpression Lysate Summary

• Description: Transient overexpression lysate of serine hydroxymethyltransferase 1 (soluble) (SHMT1), transcript variant 2 The lysate was created in HEK293T cells, using Plasmid ID RC205102 and based on accession number NM_148918. The protein contains a C-MYC/DDK Tag.
• Gene: SHMT1

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 48.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SHMT1 Overexpression Lysate

• 14 kDa protein
• CSHMT
• cytoplasmic serine hydroxymethyltransferase
• Glycine hydroxymethyltransferase
• MGC15229
• MGC24556
• serine hydroxymethyltransferase 1 (soluble)
• serine hydroxymethyltransferase, cytosolic
• Serine methylase
• SHMTEC 2.1.2.1

Background

This gene encodes the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. Additional transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]. Transcript Variant: This variant (2) lacks an in-frame exon within the coding region, as compared to variant 1. Isoform 2 is shorter than isoform 1 and has identical N- and C-termini. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SHMT1