SHMT1 Antibody (AT26E5)

Images

 
Western Blot: SHMT1 Antibody (AT26E5) [NBP2-59437] - Lane 1: A549 cell lysates, Lane 2: HeLa cell lysates, Lane 3: NIH-3T3 cell lysates, Lane 4: LnCaP cell lysates
Immunocytochemistry/ Immunofluorescence: SHMT1 Antibody (AT26E5) [NBP2-59437] - Analysis of SHMT1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SHMT1 antibody (1:100) with ...read more
Flow Cytometry: SHMT1 Antibody (AT26E5) [NBP2-59437] - Analysis of SHMT1 in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
AT26E5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SHMT1 Antibody (AT26E5) Summary

Immunogen
Recombinant human SHMT1 (1-483aa) purified from E. coli
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SHMT1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Flow Cytometry 1:10-1:1043
  • Immunocytochemistry/Immunofluorescence 1:100

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Liquid. In PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for SHMT1 Antibody (AT26E5)

  • 14 kDa protein
  • CSHMT
  • cytoplasmic serine hydroxymethyltransferase
  • Glycine hydroxymethyltransferase
  • MGC15229
  • MGC24556
  • serine hydroxymethyltransferase 1 (soluble)
  • serine hydroxymethyltransferase, cytosolic
  • Serine methylase
  • SHMTEC 2.1.2.1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SHMT1 Antibody (NBP2-59437) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SHMT1