SHMT1 Antibody (4F9) - Azide and BSA Free

Western Blot: SHMT1 Antibody (4F9) [H00006470-M01] - SHMT1 monoclonal antibody (M01), clone 4F9 Analysis of SHMT1 expression in HeLa.

Immunohistochemistry-Paraffin: SHMT1 Antibody (4F9) [H00006470-M01] - Analysis of monoclonal antibody to SHMT1 on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 1 ug/ml.

Western Blot: SHMT1 Antibody (4F9) [H00006470-M01] - Analysis of SHMT1 expression in transfected 293T cell line by SHMT1 monoclonal antibody (M01), clone 4F9.Lane 1: SHMT1 transfected lysate(53.1 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: SHMT1 Antibody (4F9) [H00006470-M01] - Analysis of monoclonal antibody to SHMT1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 1 ug/ml.

Sandwich ELISA: SHMT1 Antibody (4F9) [H00006470-M01] - Detection limit for recombinant GST tagged SHMT1 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 4F9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SHMT1 Antibody (4F9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: SHMT1 (NP_004160, 374 a.a. ~ 482 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EKVLEACSIACNKNTCPGDRSALRPSGLRLGTPALTSRGLLEKDFQKVAHFIHRGIELTLQIQSDTGVRATLKEFKERLAGDKYQAAVQALREEVESFASLFPLPGLPD
• Specificity: SHMT1 - serine hydroxymethyltransferase 1 (soluble)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SHMT1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SHMT1 Antibody (4F9) - Azide and BSA Free

• 14 kDa protein
• CSHMT
• cytoplasmic serine hydroxymethyltransferase
• EC 2.1.2.1
• Glycine hydroxymethyltransferase
• MGC15229
• MGC24556
• serine hydroxymethyltransferase 1 (soluble)
• serine hydroxymethyltransferase, cytosolic
• Serine methylase
• SHMT
• SHMT1
• SHMTEC 2.1.2.1

Background

This gene encodes the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. Additional transcript variants have been described, but their biological validity has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SHMT1 Antibody (H00006470-M01)(1)

Publication using H00006470-M01

• Genovese F, Gualandi A, Taddia L et al. Mass Spectrometric/Bioinformatic Identification of a Protein Subset That Characterizes the Cellular Activity of Anticancer Peptides. J Proteome Res 2014-09-29 [PMID: 25196676]

Bioinformatics

• Gene Symbol: SHMT1
• Entrez:
  • 6470(Human)
• OMIM:
  • 182144(Human)
• Uniprot:
  • NP_004160(Human)