SHBG Antibody (336711) [Unconjugated]

Recombinant Human SHBG protein was serially diluted 2-fold and captured by Mouse Anti-Human SHBG Monoclonal Antibody (Catalog # MAB2656) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human SHBG Monoclonal Antibody (Catalog # MAB2656) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

The catalytic activities of STT3A or STT3B are not required for DENV replication. (A) Huh7.5.1 cells were transduced with a lentivirus encoding a puromycin resistance marker, Cas9, and a single guide RNA (sgRNA) targeting the indicated genes. The sgRNAs targeting GFP act as a negative control. Two independent sgRNAs (indicated by 1 or 2 below the bars in the bar graph) were used per target gene to generate knockout cell pools. Cells were infected with luc-DENV for 3 days, and luciferase activity was measured in relative light units (RLU). Western blots for the indicated proteins are shown below the bar graph. Arrows indicate nonspecific bands. (B and C) Knockout cell pools were transduced with lentiviral vectors to rescue expression of the indicated protein. “STT3A dead” is a catalytically inactive W526A D527A double mutant. “STT3B dead” is a catalytically inactive W605A D606A double mutant with a C-terminal V5 tag. Transient transfection of constructs to express pSAP and SHBG were used to assess STT3A and STT3B catalytic activity. Western blots below the bar graph show the expression of the indicated proteins. wt and WT, wild type. (D) Knockout cell pools were transiently transfected with a luc-DENV replicon, and luciferase activity was measured at 4 h, 48 h, and 72 h posttransfection (hpt). Data are plotted as a ratio of RLU to the RLU at 4 hpt to control for transfection efficiency. GDD indicates a polymerase dead replicon with replacement of the GNN active site sequence in the NS5 RNA polymerase by GDD. (E) Cell viability for knockout cell pools was measured by Cell-Titer Glo over the course of 4 days for three independent wells. Data are normalized to day 1 values. The values for the parental Huh7.5.1 cells (P) are shown. In panels A to E, data are expressed as means plus standard deviations (SD) (error bars) for three independent biological replicates. In panels A to D, statistical significance was determined by Student’s t test and indicated by asterisks as follows: *, P < 0.05; **, P < 0.005; ***, P < 0.0005. In panel A, the means were compared to the mean for GFP sgRNA 2. In panels B and C, the means were compared to the means of their respective knockouts. In panel D, the means were compared to the mean for the GFP control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28720733), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: ELISA
• Clone: 336711
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

SHBG Antibody (336711) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human SHBG
  Leu30-His402
  Accession # P04278
• Specificity: Detects human SHBG in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SHBG
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SHBG Antibody (336711) [Unconjugated]

• ABP
• ABPTestosterone-estrogen-binding globulin
• androgen binding protein
• MGC126834
• MGC138391
• SBP
• sex hormone-binding globulin
• Sex steroid-binding protein
• SHBG
• TEBG
• Testis-specific androgen-binding protein
• testosterone-binding beta-globulin
• Testosterone-estradiol-binding globulin

Background

Human Sex Hormone Binding Globulin (SHBG), also known as ABP or androgen binding protein, is a variably glycosylated, secreted non-disulfide linked homodimer that belongs to the SHBG family. Members of this small family have tandem repeats of 170 aa long laminin a chain G-like domains. Each SHBG monomer is a 47‑53 kDa, 373 aa glycoprotein that contains one steroid-binding site in its N-terminal G-like domain. Male and female sex hormones are bound with equal affinity. SHBG is synthesized by the liver and circulates in blood; ABP (SHBG in the testis) is synthesized by Sertoli cells and circulates in the male reproductive system. Human SHBG shares approximately 67% aa sequence identity with mouse and rat SHBG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SHBG Antibody (MAB2656)(1)

Publication using MAB2656

• DL Lin, NA Cherepanov, L Bozzacco, MR MacDonald, R Gilmore, AW Tai Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex MBio, 2017-07-18;8(4):. 2017-07-18 [PMID: 28720733] (Western Blot, Human)

Review for SHBG Antibody (MAB2656) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 04/04/2020

Summary

• Application: Western Blot
• Sample Tested: hela cell
• Species: Human
• Lot: XRA031609A

Bioinformatics

• Gene Symbol: SHBG
• Uniprot:
  • P04278()