SGPL1 Antibody [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 700

SGPL1 Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human SGPL1
  Lys342-His568
  Accession # O95470
• Specificity: Detects human SGPL1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SGPL1 Antibody [Alexa Fluor® 700]

• EC 4.1.2.27
• FLJ13811
• hSPL
• KIAA1252
• S1PL
• SGPL1
• Sphingosine-1-phosphate aldolase
• sphingosine-1-phosphate lyase 1
• SPL
• SPLSPL 1
• SP-lyase 1
• SP-lyase

Background

SGPL1 (Sphingosine-1-phosphate lyase; also hSPL) is a 60‑63 kDa member of the SGPL1 subfamily, group II decarboxylase family of enzymes. It is very widely expressed, and particularly concentrated in cells undergoing rapid turnover. SGPL1 is found in the ER, and cleaves phosphorylated sphingoid bases into fatty aldehydes and phosphoethanolamine. This regulates a diversity of cellular processes such as apoptosis and T cell migration. Human SGPL1 is 568 amino acids (aa) in length. It is a type III transmembrane protein (luminal N-terminus with no signal sequence) that contains a 507 aa cytoplasmic domain (aa 62‑568). This domain contains a vitamin B6 binding site (aa 344‑364) that is part of a large catalytic region (aa 116‑506). Over aa 342‑568, human SGPL1 shares 86% aa identity with mouse SGPL1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.