SGLT1/SLC5A1 Antibody

Images

 
Western Blot: SGLT1/SLC5A1 Antibody [NBP2-27569] - Staining of isolated Brush Border Membranes from Human Kidney (75ug protein) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 ...read more
Immunocytochemistry/ Immunofluorescence: SGLT1/SLC5A1 Antibody [NBP2-27569] - Staining of cryosections of Human Kidney. Data obtained from I Vrhovac and I Sabolic, Institute for Medical Research and Occupational Health, ...read more
Immunohistochemistry-Paraffin: SGLT1/SLC5A1 Antibody [NBP2-27569] - Staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SGLT1/SLC5A1 Antibody Summary

Immunogen
Peptide with sequence C-KETIEIETQVPEKKK, from the internal region of the protein sequence according to NP_000334.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SLC5A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 3-6 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:2000
  • Immunohistochemistry-Paraffin
  • Peptide ELISA 1:4000
Application Notes
Western blot: Approx 75kDa band observed in isolated Brush Border membranes from Human Kidney (calculated MW of 73.5kDa according to NP_000334.1). Immunohistochemistry: In paraffin embedded Human Small Intestine shows staining of the glycocalix. Immunocytochemistry: PFA-fixed 4um cryosections of Human Kidney shows staining of the brush border of proximal straight tubules (S3 segments) in the outer stripe zone. The signal drops to background levels upon blocking with the immunizing peptide.

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SGLT1/SLC5A1 Antibody

  • D22S675
  • High affinity sodium-glucose cotransporter
  • Na+/glucose cotransporter 1
  • NAGT
  • NAGTsodium/glucose cotransporter 1
  • SGLT1
  • SGLT1Na(+)/glucose cotransporter 1
  • SLC5A1
  • solute carrier family 5 (sodium/glucose cotransporter), member 1
  • Solute carrier family 5 member 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SLC5A1
Entrez
Uniprot