SFRS7 Antibody

Western Blot: SFRS7 Antibody [NBP2-94049] - Western blot analysis of extracts of various cell lines, using SFRS7 Rabbit pAb (NBP2-94049) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunocytochemistry/Immunofluorescence: SFRS7 Antibody [NBP2-94049] - Analysis of U-2 OS cells using SFRS7 at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: SFRS7 Antibody [NBP2-94049] - Immunohistochemistry of paraffin-embedded mouse testis using SFRS7 Rabbit pAb (NBP2-94049) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: SFRS7 Antibody [NBP2-94049] - Immunohistochemistry of paraffin-embedded human esophageal using SFRS7 Rabbit pAb (NBP2-94049) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SFRS7 Antibody Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-120 of human SRSF7 (NP_001026854).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SRSF7
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for SFRS7 Antibody

• AAG3
• aging-associated protein 3
• arginine/serine-rich 7, 35kDa
• HSSG1
• RBM37
• serine/arginine-rich splicing factor 7
• SFRS7ZCRB2,9G8
• Splicing factor 9G8
• splicing factor, arginine/serine-rich 7 (35kD)
• Splicing factor, arginine/serine-rich 7
• SR splicing factor 7
• ZCCHC20

Background

SFRS7 is required for pre-mRNA splicing. Can also modulate alternative splicing in vitro

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SRSF7