sFRP-2 Antibody (331022) [Alexa Fluor® 594]

• Reactivity: Mu
• Applications: WB
• Clone: 331022
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 594

sFRP-2 Antibody (331022) [Alexa Fluor® 594] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse sFRP-2
  Leu25-Cys295
  Accession # P97299
• Specificity: Detects mouse sFRP-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) sFRP-1, recombinant mouse sFRP-3, or rhsFRP-4.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for sFRP-2 Antibody (331022) [Alexa Fluor® 594]

• FRP2
• FRP-2secreted apoptosis related protein 1
• SARP-1
• SARP1sFRP-2
• SDF-5
• Secreted apoptosis-related protein 1
• secreted frizzled-related protein 2
• sFRP2
• sFRP-2

Background

Secreted Frizzled Related Proteins (sFRPs) are a family of vertebrate proteins which contain homology to the ligand-binding domain of the Frizzled family of transmembrane receptors. The sFRPs are approximately 30-35 kDa in size and are comprised of 3 domains: a signal sequence; a cysteine-rich domain (CRD) of about 110 amino acids (aa) with a high degree of similarity to the Frizzled proteins, including 10 conserved cysteines, and a 175 aa conserved hydrophilic carboxy terminal region. Because sFRPs contain a CRD very similar to the region responsible for binding Wnt ligands in Frizzleds, sFRPS are thought to act as soluble antagonists of Wnt signals. sFRP-2, also known as SARP-1, SDF-5, and FRP-2, is expressed during mouse embryogenesis in the eye, brain, neural tube, craniofacial mesenchyme, joints, testis, pancreas, kidney, and regions of smooth muscle cell development. Expression in the adult animal has been detected in the eye, heart, lung, along with preadipose and adipose tissues in mice and humans. Mouse and human sFRP-2 proteins share 98% aa identity, and related proteins have also been identified in chick, rabbit, and frog. A variety of activities have been reported for sFRP-2 including an increased resistance to apoptosis, antagonism of Xwnt -8 signaling in Xenopus, regulation of Wnt-4 signaling (with sFRP-1) in renal organogenesis, and inhibition of the migration of glioma cells (1-7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P97299()