sFRP-1 Antibody

Images

 
Western Blot: sFRP-1 Antibody [NB600-499] - Analysis of a band 37 kDa (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells. Lysates were run on a SDS-PAGE and transferred onto ...read more
Immunohistochemistry: sFRP-1 Antibody [NB600-499] - Used at a 1:800 dilution for 20 min to detect SFRP in human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated antigen retrieval ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

sFRP-1 Antibody Summary

Immunogen
Synthetic peptide: IGPYQSGRFYTKC, corresponding to amino acids 42 - 53 of Human SFRP1.
Epitope
aa 42-53
Specificity
SFRP1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SFRP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:2000
  • ELISA 1:5000-1:25000
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry 1:800
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Expect a band approximately 37 kDa in size corresponding - Sfrp1 by western blotting in the appropriate cell lysate or extract.
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross-reacts with Human. Expected to cross-react with Mouse (91% identity with immunogen) due to sequence homology. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for sFRP-1 Antibody

  • FRP1
  • FRP-1FrzA
  • FRPSARP-2
  • FrzA
  • SARP-2
  • SARP2sFRP-1
  • Secreted apoptosis-related protein 2
  • secreted frizzled-related protein 1
  • sFRP1
  • sFRP-1

Background

SFRP1 (also known as FRP, FRP1, SARP2, Secreted Apoptosis-related Protein 2, Secreted Frizzledrelated Protein and Secreted frizzled-related protein 1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

1169-FR/CF
Species: Mu
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
NBP2-20331
Species: Hu
Applications: WB, IHC, IHC-P
1827-SF/CF
Species: Hu
1341-WF/CF
Species: Hu
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF4885
Species: Mu
Applications: WB, IP
AF592
Species: Mu
Applications: WB, IHC
NBP2-03644
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF1118
Species: Hu
Applications: WB, IHC
NBP2-32840
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC-P, IP
NBP2-36491
Species: Hu
Applications: WB, Flow, IHC, IHC-P, Flow-CS
AF748
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP2-50465
Species: Hu
Applications: Flow, IHC, CyTOF-ready, Flow-CS
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
NB600-499
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for sFRP-1 Antibody (NB600-499) (0)

There are no publications for sFRP-1 Antibody (NB600-499).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for sFRP-1 Antibody (NB600-499) (0)

There are no reviews for sFRP-1 Antibody (NB600-499). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for sFRP-1 Antibody (NB600-499). (Showing 1 - 1 of 1 FAQ).

  1. May I request confirmation that SFRP1 Antibody (NB600-499) has been tested in ELISA using cell lysate as sample instead of immunogen as sample? Will this antibody detect endogenous levels of the protein in ELISA? We have a customer who has had sensitivity issues with other SFRP1 antibodies and is looking for one that can detect endogenous levels of the protein. Thanks for your help. (This product lists ELISA as an application, what samples were used and can the endogenous protein be detected by ELISA?)
    • I have pulled up information on this product and it looks like it has been tested in ELISA on the immunogen as opposed to an endogenous cell lysate. However, we do have a guarantee policy and we have confirmed this antibody to detect properly in Western Blot with cell lysate containing endogenous levels of the protein so it should also have an affinity for the protein in ELISA as well. (We have tested this SFRP1 antibody in ELISA using the native full length protein. While we have confirmed the presence of endogenous protein in cell lysate samples via western blot, we have not used cell lysate samples for detection in ELISA. Due to the western blot results, we predict that cell lysates containing endogenous levels of the SFRP1 protein should bind and detect the antibody.)

Secondary Antibodies

 

Isotype Controls

Additional sFRP-1 Products

Bioinformatics Tool for sFRP-1 Antibody (NB600-499)

Discover related pathways, diseases and genes to sFRP-1 Antibody (NB600-499). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for sFRP-1 Antibody (NB600-499)

Discover more about diseases related to sFRP-1 Antibody (NB600-499).
 

Pathways for sFRP-1 Antibody (NB600-499)

View related products by pathway.

PTMs for sFRP-1 Antibody (NB600-499)

Learn more about PTMs related to sFRP-1 Antibody (NB600-499).
 

Research Areas for sFRP-1 Antibody (NB600-499)

Find related products by research area.

Blogs on sFRP-1

There are no specific blogs for sFRP-1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our sFRP-1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol SFRP1
Entrez