sFRP-1 Antibody


Western Blot: sFRP-1 Antibody [NB600-499] - Analysis of a band 37 kDa (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells. Lysates were run on a SDS-PAGE and transferred onto more
Immunohistochemistry: sFRP-1 Antibody [NB600-499] - Used at a 1:800 dilution for 20 min to detect SFRP in human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated antigen retrieval more

Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
1 mg/ml

Order Details

sFRP-1 Antibody Summary

SFRP1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a 12 aa region of human sFRP-1 protein.
aa 42-53
Immunogen affinity purified
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  • Western Blot 1:200-1:2000
  • ELISA 1:5000-1:15000
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry 1:800
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Expect a band approximately 37 kDa in size corresponding - Sfrp1 by western blotting in the appropriate cell lysate or extract.
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross-reacts with Human. Expected to cross-react with Mouse (91% identity with immunogen) due to sequence homology. Not yet tested in other species.

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% Sodium Azide
1 mg/ml
Immunogen affinity purified

Alternate Names for sFRP-1 Antibody

  • FRP1
  • FRP-1FrzA
  • FrzA
  • SARP-2
  • SARP2sFRP-1
  • Secreted apoptosis-related protein 2
  • secreted frizzled-related protein 1
  • sFRP1
  • sFRP-1


SFRP1 (also known as FRP, FRP1, SARP2, Secreted Apoptosis-related Protein 2, Secreted Frizzledrelated Protein and Secreted frizzled-related protein 1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for sFRP-1 Antibody (NB600-499). (Showing 1 - 1 of 1 FAQ).

  1. May I request confirmation that SFRP1 Antibody (NB600-499) has been tested in ELISA using cell lysate as sample instead of immunogen as sample? Will this antibody detect endogenous levels of the protein in ELISA? We have a customer who has had sensitivity issues with other SFRP1 antibodies and is looking for one that can detect endogenous levels of the protein. Thanks for your help. (This product lists ELISA as an application, what samples were used and can the endogenous protein be detected by ELISA?)
    • I have pulled up information on this product and it looks like it has been tested in ELISA on the immunogen as opposed to an endogenous cell lysate. However, we do have a guarantee policy and we have confirmed this antibody to detect properly in Western Blot with cell lysate containing endogenous levels of the protein so it should also have an affinity for the protein in ELISA as well. (We have tested this SFRP1 antibody in ELISA using the native full length protein. While we have confirmed the presence of endogenous protein in cell lysate samples via western blot, we have not used cell lysate samples for detection in ELISA. Due to the western blot results, we predict that cell lysates containing endogenous levels of the SFRP1 protein should bind and detect the antibody.)

Secondary Antibodies


Isotype Controls

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Gene Symbol SFRP1