sFRP-1 Antibody - BSA Free

Western Blot: sFRP-1 Antibody [NB600-499] - Analysis of a band 37 kDa (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells. Lysates were run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:230 dilution of anti-SFRP1 antibody overnight at 4C. Signal was detected using standard techniques. Personnel communication Becky Mercer, University of Columbia.

Immunohistochemistry: sFRP-1 Antibody [NB600-499] - Used at a 1:800 dilution for 20 min to detect SFRP in human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated antigen retrieval prior to blocking. HRP Gt-a-Rabbit IgG is suitable for secondary antibody detection.

Affinity Purified anti-Human SFRP1 antibody was used at a 1:800 dilution for 20 min to detect SFRP in human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated antigen retrieval prior to blocking. HRP Gt-a-Rabbit IgG

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

sFRP-1 Antibody - BSA Free Summary

• Description: This affinity purified antibody is directed against human SFRP protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: sFRP-1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a 12 aa region of human Sfrp1 protein. (Uniprot: Q8N474)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SFRP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:15000
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Immunohistochemistry 1:800
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:200-1:2000
• Application Notes: This affinity purified antibody has been tested for use in ELISA, western blot, and IHC. This antibody is suitable for immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Sfrp1 by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. Expect cross reactivity with sFRP-1 from mouse, rat and bovine sources as only a single amino acid residue change is found within the immunogen sequence (91% positive by BLAST). Cross reactivity with sFRP-1 homologues from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for sFRP-1 Antibody - BSA Free

• FRP1
• FRP-1FrzA
• FRPSARP-2
• FrzA
• SARP-2
• SARP2sFRP-1
• Secreted apoptosis-related protein 2
• secreted frizzled-related protein 1
• sFRP1
• sFRP-1

Background

SFRP1 (also known as FRP, FRP1, SARP2, Secreted Apoptosis-related Protein 2, Secreted Frizzledrelated Protein and Secreted frizzled-related protein 1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for sFRP-1 Antibody (NB600-499). (Showing 1 - 1 of 1 FAQ).

1. May I request confirmation that SFRP1 Antibody (NB600-499) has been tested in ELISA using cell lysate as sample instead of immunogen as sample? Will this antibody detect endogenous levels of the protein in ELISA? We have a customer who has had sensitivity issues with other SFRP1 antibodies and is looking for one that can detect endogenous levels of the protein. Thanks for your help. (This product lists ELISA as an application, what samples were used and can the endogenous protein be detected by ELISA?)
   • I have pulled up information on this product and it looks like it has been tested in ELISA on the immunogen as opposed to an endogenous cell lysate. However, we do have a guarantee policy and we have confirmed this antibody to detect properly in Western Blot with cell lysate containing endogenous levels of the protein so it should also have an affinity for the protein in ELISA as well. (We have tested this SFRP1 antibody in ELISA using the native full length protein. While we have confirmed the presence of endogenous protein in cell lysate samples via western blot, we have not used cell lysate samples for detection in ELISA. Due to the western blot results, we predict that cell lysates containing endogenous levels of the SFRP1 protein should bind and detect the antibody.)

Bioinformatics

• Gene Symbol: SFRP1
• Entrez:
  • 6422(Human)
• Uniprot:
  • Q8N474(Human)