SF3A3 Antibody

Western Blot: SF3A3 Antibody [NBP2-20325] - Sample (30 ug of whole cell lysate) A: NIH-3T3 7. 5% SDS PAGE gel, diluted at 1:1000.

Western Blot: SF3A3 Antibody [NBP2-20325] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Bv, Pm, Xp
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SF3A3 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human SF3A3. The exact sequence is proprietary.
• Localization: Nucleus speckle
• Predicted Species: Rat (100%), Primate (100%), Xenopus (90%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SF3A3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (100%), Xenopus laevis (90%), Zebrafish (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SF3A3 Antibody

• PRP9
• PRPF9
• SAP 61
• SAP61splicing factor 3a, subunit 3, 60kD
• SF3a60pre-mRNA splicing factor SF3a (60kD)
• spliceosome associated protein 61
• Spliceosome-associated protein 61
• splicing factor 3A subunit 3
• splicing factor 3a, subunit 3, 60kDa

Background

This gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP. This gene has a pseudogene on chromosome 20. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SF3A3
• Uniprot:
  • Q12874(Human)