SF20/MYDGF Antibody (2562A) [Unconjugated]

Western blot shows lysates of HT-2 mouse T cell line, NIH-3T3 mouse embryonic fibroblast cell line, DU145 human prostate carcinoma cell line, NRK rat normal kidney cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat SF20/MYDGF Monoclonal Antibody (Catalog # MAB1104) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SF20/MYDGF at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Tramp-C1 mouse cell line was stained with Rabbit Anti-Human/Mouse/Rat SF20/MYDGF Monoclonal Antibody (Catalog # MAB1104, filled histogram) or Rabbit IgG isotype control antibody (Catalog # MAB1050, open histogram) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.

SF20/MYDGF was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rabbit Anti-Mouse SF20/MYDGF Monoclonal Antibody (Catalog # MAB1104) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SF20/MYDGF was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human/Mouse/Rat SF20/MYDGF Monoclonal Antibody (Catalog # MAB1104) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of HT‑2 mouse T cell line and Rat‑2 rat embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for SF20/MYDGF at approximately 22 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human/Mouse/Rat SF20/MYDGF Monoclonal Antibody (Catalog # MAB1104) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC, CyTOF-ready, Flow, ICC/IF
• Clone: 2562A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

SF20/MYDGF Antibody (2562A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse myeloma cell line NS0-derived mouse SF20/MYDGF
  Val25-Leu166
  Accession # Q9CPT4
• Specificity: Detects mouse SF20/MYDGF in direct ELISAs. Detects human, mouse, and rat SF20/MYDGF in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MYDGF
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 0.3-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SF20/MYDGF Antibody (2562A) [Unconjugated]

• C19orf10
• chromosome 19 open reading frame 10
• EUROIMAGE1875335
• IL25
• IL27
• IL27w
• interleukin 27 working designation
• interleukin-25
• Ly6elg
• MYDGF
• myeloid-derived growth factor
• R33729_1
• SF20
• Stromal cell-derived growth factor SF20
• UPF0556 protein C19orf10

Background

Myeloid-Derived Growth Factor, or MYDGF, is a Bone marrow-derived monocyte protein, and it is correlated with enhanced metabolic activity, suppression of apoptosis, and stimulation of cell proliferation (1). MYDGF is expressed predominantly in inflammatory cells, such as monocytes and macrophages (1). Up-regulation of MYDGF expression was also found during adipocyte differentiation (2). Expression of MYDGF was induced in the circulation and heart tissue after myocardial infarction. It promotes cardiac myocyte survival by stimulating endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway, and inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway (1). MYDGF was found over-expressed in approximately two-thirds of Hepatocellular Carcinoma (HCC) tissues, and its expression was significantly positively correlated with that of alpha-fetoprotein (AFP) (3). In HCC, MYDGF could regulate cell proliferation through activating Akt/mitogen-activated protein kinase pathways (3). Mouse MYDGF shares 92% amino acid sequence identity with both human and rat MYDGF. Intriguingly, virtually all homologs of MYDGF have a C-terminal putative ER retention sequence BXEL (B: Arg, His, or Lys; X: variable residue; E: Glu; L: Leu), which has the potential to retain human MYDGF and its homologs in the ER, whereas truncated MYDGF without BXEL is secreted from the cell (4). However, the functions of these different forms remain unclear.

1. Korf-Klingebiel, M. et al. (2015) Nat. Med. 10:3778.
2. Wang, P. et al. (2004) Cell. Mol. Life Sci. 61:2405.
3. Sunagozaka, H. et al. (2011) Int. J. Cancer 129:1576.
4. Bortnov, V. et al. (2018) J. Biol. Chem. 293:13166.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYDGF
• Uniprot:
  • Q9CPT4()