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HAF008). A specific band was detected for SF20/MYDGF at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
MAB1050, open histogram) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. View our protocol for Staining Intracellular Molecules." class="big_lightbox" target="_blank">
VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
HAF017). A specific band was detected for SF20/MYDGF at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
HAF018). A specific band was detected for SF20/MYDGF at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. View our protocol for Staining Intracellular Molecules." class="big_lightbox" target="_blank">
Species: Hu Applications: WB, CyTOF-ready, Flow Host: Mouse Monoclonal
Myeloid-Derived Growth Factor, or MYDGF, is a Bone marrow-derived monocyte protein, and it is correlated with enhanced metabolic activity, suppression of apoptosis, and stimulation of cell proliferation (1). MYDGF is expressed predominantly in inflammatory cells, such as monocytes and macrophages (1). Up-regulation of MYDGF expression was also found during adipocyte differentiation (2). Expression of MYDGF was induced in the circulation and heart tissue after myocardial infarction. It promotes cardiac myocyte survival by stimulating endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway, and inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway (1). MYDGF was found over-expressed in approximately two-thirds of Hepatocellular Carcinoma (HCC) tissues, and its expression was significantly positively correlated with that of alpha-fetoprotein (AFP) (3). In HCC, MYDGF could regulate cell proliferation through activating Akt/mitogen-activated protein kinase pathways (3). Mouse MYDGF shares 92% amino acid sequence identity with both human and rat MYDGF. Intriguingly, virtually all homologs of MYDGF have a C-terminal putative ER retention sequence BXEL (B: Arg, His, or Lys; X: variable residue; E: Glu; L: Leu), which has the potential to retain human MYDGF and its homologs in the ER, whereas truncated MYDGF without BXEL is secreted from the cell (4). However, the functions of these different forms remain unclear.
![Western Blot SF20/MYDGF Antibody (2562A) [Unconjugated]](http://images.novusbio.com/images/antibody/SF20_MAB1104_Western_Blot_24109.jpg)
![Western Blot SF20/MYDGF Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/SF20_AF1147_Western_Blot_16836.jpg)
![Western Blot SF20/MYDGF Antibody (1009420) [Unconjugated]](http://images.novusbio.com/images/antibody/SF20_MAB1147_Western_Blot_23484.jpg)
![Intracellular Staining by Flow Cytometry SF20/MYDGF Antibody (2562A) [Unconjugated]](http://images.novusbio.com/images/antibody/SF20_MAB1104_Immunocytochemistry__Immunofluorescence_24015.jpg)
![Immunocytochemistry SF20/MYDGF Antibody (2562A) [Unconjugated]](http://images.novusbio.com/images/antibody/SF20_MAB1104_Immunocytochemistry__Immunofluorescence_24150.jpg)
![Immunohistochemistry SF20/MYDGF Antibody (2562A) [Unconjugated]](http://images.novusbio.com/images/antibody/SF20_MAB1104_Immunohistochemistry_24283.jpg)
![Simple Western SF20/MYDGF Antibody (2562A) [Unconjugated]](http://images.novusbio.com/fullsize/antibody/SF20_MAB1104_Simple_Western_24238.jpg)
![Simple Western SF20/MYDGF Antibody [Unconjugated]](http://images.novusbio.com/fullsize/antibody/af1147_human-sf20-affinity-purified-polyclonal-ab-simple-western-41202395451.jpg)
![Western Blot SF20/MYDGF Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1147_human-sf20-affinity-purified-polyclonal-ab-western-blot-12122025549916.jpg)
![Western Blot SF20/MYDGF Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1147_human-sf20-affinity-purified-polyclonal-ab-western-blot-12122025552279.jpg)
![Western Blot SF20/MYDGF Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1147_human-sf20-affinity-purified-polyclonal-ab-western-blot-12122025751212.jpg)
![Western Blot SF20/MYDGF Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1147_human-sf20-affinity-purified-polyclonal-ab-western-blot-121220257481319.jpg)
![Intracellular Staining by Flow Cytometry SF20/MYDGF Antibody (1009420) [Unconjugated]](http://images.novusbio.com/images/antibody/SF20_MAB1147_Flow_Cytometry_23483.jpg)
![Binding Activity SF20/MYDGF [Unconjugated]](http://images.novusbio.com/images/protein/SF20_10231MY_2872.jpg)
![SDS-Page SF20/MYDGF [Unconjugated]](http://images.novusbio.com/fullsize/protein/SF20_10231MY_2644.jpg)
![Binding Activity SF20/MYDGF [Unconjugated]](http://images.novusbio.com/images/protein/SF20_10232MY_2873.jpg)
![SDS-Page SF20/MYDGF [Unconjugated]](http://images.novusbio.com/fullsize/protein/SF20_10232MY_2645.jpg)
