SETD7 Antibody

Western Blot: SETD7 Antibody [NBP2-20319] - Sample (30 ug of whole cell lysate) A: A549 B: HeLa 10% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, RM
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SETD7 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human SETD7. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Mouse (96%), Rat (97%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SETD7
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SETD7 Antibody

• EC 2.1.1.364
• EC 2.1.1.43
• H3-K4-HMTase SETD7
• histone H3-lysine 4-specific methyltransferase
• histone-lysine N-methyltransferase SETD7
• KIAA1717
• KIAA1717SET domain-containing protein 7
• KMT7
• KMT7FLJ21193
• Lysine N-methyltransferase 7
• SET domain containing (lysine methyltransferase) 7
• SET7
• SET7/9
• SET7/9Histone H3-K4 methyltransferase SETD7
• SET7Set9
• SET9
• SETD7

Background

Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SETD7
• Uniprot:
  • Q8WTS6(Human)