SETD1A Antibody - BSA Free

Western Blot: SETD1A Antibody [NBP3-12907] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SETD1A antibody (NBP3-12907) diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: SETD1A Antibody [NBP3-12907] - SETD1A antibody detects SETD1A protein at nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SETD1A protein stained by SETD1A antibody (NBP3-12907) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: SETD1A Antibody [NBP3-12907] - Mouse brain. SETD1A antibody (NBP3-12907) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: SETD1A Antibody [NBP3-12907] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SETD1A antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SETD1A Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human SETD1A. The exact sequence is proprietary.
• Predicted Species: Bovine (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SETD1A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 186 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for SETD1A Antibody - BSA Free

• histone-lysine N-methyltransferase SETD1A
• hSET1A
• KIAA0339EC 2.1.1.43
• KMT2FSET1
• Lysine N-methyltransferase 2F
• SET domain containing 1A
• SET domain-containing protein 1A
• Set1
• Set1/Ash2 histone methyltransferase complex subunit SET1
• SET1A

Background

SET1A is a component of a histone methyltransferase (HMT) complex that produces mono-, di-, and trimethylated histone H3 at Lys4. The complex is the analog of the S. cerevisiae Set1/COMPASS complex (Lee and Skalnik, 2005 [PubMed 16253997]). Also see SET1B (MIM 611055).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SETD1A