SETD1A Antibody (BLR024E)

Western Blot: SETD1A Antibody (BLR024E) [NBP2-76401] - Detection of human and mouse hSET1 by WB of HeLa, HEK293T, Jurkat, and TCMK-1 lysate. Antibody: Rabbit anti-hSET1 recombinant monoclonal [BLR024E] (NBP2-76401). Secondary: HRP-conjugated goat anti-rabbit IgG.

Immunohistochemistry-Paraffin: SETD1A Antibody (BLR024E) [NBP2-76401] - Section of human lung carcinoma. Antibody: Rabbit anti-hSET1 recombinant monoclonal antibody [BLR024E] used at 1:25. Secondary: HRPconjugated goat anti-rabbit IgG. (Substrate: DAB).

Immunofluorescence: SETD1A Antibody (BLR024E) [NBP2-76401] - Detection of human hSET1 (red) in FFPE ovarian carcinoma by IHC-IF. Antibody: Rabbit anti-hSET1 recombinant monoclonal [BLR024E] (NBP2-76401). Secondary:DyLight 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).

Immunoprecipitation: SETD1A Antibody (BLR024E) [NBP2-76401] - Detection of human hSET1 by WB of immunoprecipitates from HEK293T lysate. Antibodies: Rabbit anti-hSET1 recombinant monoclonal [BLR024E] (NBP2-76401) and another rabbit anti-hSET1 antibody.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP
• Clone: BLR024E
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

SETD1A Antibody (BLR024E) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Immunogen sequence between amino acids 1200 and 1250
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SETD1A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:25-1:250
  • Immunohistochemistry 1:25 - 1:250
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 20 ul/mg lysate
  • Western Blot 1:1000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

This Product sold under License from Bethyl Laboratories, Inc.

Alternate Names for SETD1A Antibody (BLR024E)

• histone-lysine N-methyltransferase SETD1A
• hSET1A
• KIAA0339EC 2.1.1.43
• KMT2FSET1
• Lysine N-methyltransferase 2F
• SET domain containing 1A
• SET domain-containing protein 1A
• Set1
• Set1/Ash2 histone methyltransferase complex subunit SET1
• SET1A

Background

hSET1 is a subunit of a human Set1-like histone methyltransferase (HMT) complex. The Set-1 like complexes are responsible for mono-, di-, and tri-methylation of histone H3 K4. The Set class of proteins takes its name from the 130-140 aa motif SET domain identified in drosophila proteins. hSET1 associates with a multitude of proteins in a Set1-like complex which includes hSet1, Ash2L, RBBP5,WDR5, hDPY-30, hSwd2, CXXC1, and HCF1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SETD1A